Please use this identifier to cite or link to this item:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

AguR, a Transmembrane Transcription Activator of the Putrescine Biosynthesis Operon in Lactococcus lactis, Acts in Response to the Agmatine Concentration

AuthorsLinares, Daniel M. CSIC; Río Lagar, Beatriz del CSIC ORCID ; Redruello, Begoña CSIC ORCID; Ladero Losada, Víctor Manuel CSIC ORCID ; Martín, M. Cruz CSIC ORCID ; Jong, Anne de; Kuipers, O. P.; Fernández García, María CSIC ORCID ; Álvarez González, Miguel Ángel CSIC ORCID
Issue Date26-Jun-2015
PublisherAmerican Society for Microbiology
CitationApplied and Environmental Microbiology 81(18): 6145-6157 (2015)
AbstractDairy industry fermentative processes mostly use Lactococcus lactis as a starter. However, some dairy L. lactis strains produce putrescine, a biogenic amine that raises food safety and spoilage concerns, via the agmatine deiminase (AGDI) pathway. The enzymatic activities responsible for putrescine biosynthesis in this bacterium are encoded by the AGDI gene cluster. The role of the catabolic genes aguB, aguD, aguA, and aguC has been studied, but knowledge regarding the role of aguR (the first gene in the cluster) remains limited. In the present work, aguR was found to be a very low level constitutively expressed gene that is essential for putrescine biosynthesis and is transcribed independently of the polycistronic mRNA encoding the catabolic genes (aguBDAC). In response to agmatine, AguR acts as a transcriptional activator of the aguB promoter (PaguB), which drives the transcription of the aguBDAC operon. Inverted sequences required for PaguB activity were identified by deletion analysis. Further work indicated that AguR is a transmembrane protein which might function as a one-component signal transduction system that senses the agmatine concentration of the medium and, accordingly, regulates the transcription of the aguBDAC operon through a C-terminal cytoplasmic DNA-binding domain typically found in LuxR-like proteins.
Publisher version (URL)
Identifiersissn: 0099-2240
e-issn: 1098-5336
Appears in Collections:(IPLA) Artículos

Files in This Item:
File Description SizeFormat
AguR_transmembrane_Linares.pdf420,68 kBAdobe PDFThumbnail
Show full item record
Review this work

Google ScholarTM




WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.