English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/125346
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Aging and metabolic control come to terms

AutorMonsalve, María
Fecha de publicación2013
CitaciónGEIRLI 2013
ResumenSirT1 is a class III histone deacetylase that has been implicated in metabolic and ROS control. In the vasculature it has been shown to decrease endothelial superoxide production, prevent endothelial dysfunction and atherosclerosis. However, the mechanisms that mediate SirT1 antioxidant functions remain to be characterized. The transcription factor FoxO3a and the transcriptional coactivator peroxisome proliferator activated receptor g-coactivator 1α(PGC- 1α) have been shown to induce the expression of antioxidant genes and to be deacetylated by SirT1. Here we investigated SirT1 regulation of antioxidant genes and the roles played by FoxO3a and PGC-1α in this regulation. [Results]: We found that SirT1 regulates the expression of several antioxidant genes in BAEC, including Mn superoxide dismutase (MnSOD), catalase, peroxiredoxins 3 and 5 (Prx3, Prx5), thioredoxin 2 (Trx2), thioredoxin reductase 2 (TR2) and uncoupling protein 2 (UCP-2) and can be localized in the regulatory regions of these genes. We also found that knockdown of either FoxO3a or PGC-1α prevented the induction of antioxidant genes by SirT1 over-expression. Furthermore, SirT1 increased the formation of a FoxO3a/PGC-1acomplex as determined by co-immunoprecipitation (IP) assays, concomitantly reducing H2O2 dependent FoxO3a and PGC-1aacetylation. Data showing that FoxO3a knockdown increases PGC-1α acetylation levels and /vice versa/, suggests that SirT1 activity on FoxO3a and PGC-1a may be dependent of the formation of a FoxO3a/PGC-1α complex. [Conclusion]: We show that SirT1 regulation of antioxidant genes in vascular endothelial cells depends on the formation of a FoxO3a/PGC-1α complex.
DescripciónResumen del trabajo presentado a la VIII Reunión del Grupo Español de Investigación en Radicales Libres, celebrado en la Facultad de Medicina de la Universidad de Valencia del 3 al 5 de junio de 2013.
URIhttp://hdl.handle.net/10261/125346
Aparece en las colecciones: (IIBM) Comunicaciones congresos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.