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dc.contributor.authorTort, Núriaes_ES
dc.contributor.authorSalvador, Juan Pabloes_ES
dc.contributor.authorAviñó, Annaes_ES
dc.contributor.authorEritja Casadellà, Ramónes_ES
dc.contributor.authorComelles, Jordies_ES
dc.contributor.authorMartínez, Elenaes_ES
dc.contributor.authorSamitier, Josepes_ES
dc.contributor.authorMarco, María Pilares_ES
dc.identifier.citationBioconjugate Chemistryes_ES
dc.description.abstractThe excellent self-assembling properties of DNA and the excellent specificity of the antibodies to detect analytes of small molecular weight under competitive conditions have been combined in this study. Three oligonucleotide sequences (N1up, N2up, and N3up) have been covalently attached to three steroidal haptens (8, hG, and 13) of three anabolic-androgenic steroids (AAS), stanozolol (ST), tetrahydrogestrinone (THG), and boldenone (B), respectively. The synthesis of steroid-oligonucleotide conjugates has been performed by the reaction of oligonucleotides carrying amino groups with carboxyl acid derivatives of steroidal haptens. Due to the chemical nature of the steroid derivatives, two methods for coupling the haptens and the ssDNA have been studied: a solid-phase coupling strategy and a solution-phase coupling strategy. Specific antibodies against ST, THG, and B have been used in this study to asses the possibility of using the self-assembling properties of the DNA to prepare biofunctional SPR gold chips based on the immobilization of haptens, by hybridization with the complementary oligonucleotide strands possessing SH groups previously immobilized. The capture of the steroid-oligonucleotide conjugates and subsequent binding of the specific antibodies can be monitored on the sensogram due to variations produced on the refractive index on top of the gold chip. The resulting steroid-oligonucleotide conjugates retain the hybridization and specific binding properties of oligonucleotides and haptens as demonstrated by thermal denaturation experiments and surface plasmon resonance (SPR).es_ES
dc.description.sponsorshipThis work was supported by grants from the Ministry of Science and Innovation, MICINN (MAT2011-29335-C03-01 and CTQ2010-20541-C03-01), CCEE (FUNMOL, FP7-NMP-213382-2), Generalitat de Catalunya (2009/SGR/1343, 2009/SGR/208), and CIBER-BBN. CIBER-BBN is an initiative funded by the VI National R&D&i Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions and financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund. Núria Tort has a FI_B fellowship from the AGAUR (Agència de Gestió d’Ajuts Universitaris i de Recerca) of the (Generalitat de Catalunya) Government of Catalonia.es_ES
dc.publisherAmerican Chemical Societyes_ES
dc.subjectBiosensing Techniqueses_ES
dc.subjectMolecular Structurees_ES
dc.subjectSurface Plasmon Resonancees_ES
dc.titleSynthesis of steroid-oligonucleotide conjugates for a DNA site-encoded SPR immunosensores_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionDOI: 10.1021/bc300138pes_ES
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