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Title

Synthesis of steroid-oligonucleotide conjugates for a DNA site-encoded SPR immunosensor

AuthorsTort, Núria; Salvador, Juan Pablo; Aviñó, Anna; Eritja Casadellà, Ramón; Comelles, Jordi; Martínez, Elena; Samitier, Josep; Marco, María Pilar
KeywordsBiosensing Techniques
DNA
Oligonucleotide
hapten
Molecular Structure
Surface Plasmon Resonance
steroid
Issue DateNov-2012
PublisherAmerican Chemical Society
CitationBioconjugate Chemistry
AbstractThe excellent self-assembling properties of DNA and the excellent specificity of the antibodies to detect analytes of small molecular weight under competitive conditions have been combined in this study. Three oligonucleotide sequences (N1up, N2up, and N3up) have been covalently attached to three steroidal haptens (8, hG, and 13) of three anabolic-androgenic steroids (AAS), stanozolol (ST), tetrahydrogestrinone (THG), and boldenone (B), respectively. The synthesis of steroid-oligonucleotide conjugates has been performed by the reaction of oligonucleotides carrying amino groups with carboxyl acid derivatives of steroidal haptens. Due to the chemical nature of the steroid derivatives, two methods for coupling the haptens and the ssDNA have been studied: a solid-phase coupling strategy and a solution-phase coupling strategy. Specific antibodies against ST, THG, and B have been used in this study to asses the possibility of using the self-assembling properties of the DNA to prepare biofunctional SPR gold chips based on the immobilization of haptens, by hybridization with the complementary oligonucleotide strands possessing SH groups previously immobilized. The capture of the steroid-oligonucleotide conjugates and subsequent binding of the specific antibodies can be monitored on the sensogram due to variations produced on the refractive index on top of the gold chip. The resulting steroid-oligonucleotide conjugates retain the hybridization and specific binding properties of oligonucleotides and haptens as demonstrated by thermal denaturation experiments and surface plasmon resonance (SPR).
Publisher version (URL)DOI: 10.1021/bc300138p
URIhttp://hdl.handle.net/10261/125337
DOI10.1021/bc300138p
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