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Título: | Functionalization of surfaces with synthetic oligonucleotides |
Autor: | Manning, Brendan CSIC; Eritja Casadellà, Ramón CSIC ORCID | Palabras clave: | Biosensors DNA Microarrays Oligonucleotides Surface analysis Nucleic Acids |
Fecha de publicación: | 2012 | Editor: | Humana Press | Citación: | Methods in Molecular Biology | Resumen: | There is a large interest in the use of nucleic acids covalently bound to surfaces for a variety of biomedical uses: biosensors, microarrays, drug delivery, lab-on-chip devices, and gene therapy, etc. Most of these applications require the covalent attachment of oligonucleotides via specific reactive groups on both modified oligonucleotide and/or surface. The purpose of this chapter is to provide experimental protocols for the synthesis of oligonucleotides and for the immobilization of these synthetic oligonucleotides onto surfaces such as gold and silicon oxide. | Versión del editor: | DOI: 10.1007/978-1-61779-388-2_6 | URI: | http://hdl.handle.net/10261/125220 | DOI: | 10.1007/978-1-61779-388-2_6 |
Aparece en las colecciones: | (IQAC) Artículos |
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Regenerative2012.pdf | Artículo principal | 1,23 MB | Adobe PDF | Visualizar/Abrir |
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