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Title

Fluorescence site-encoded DNA addressable hapten microarray for anabolic androgenic steroids

AuthorsTort, Núria; Salvador, Juan Pablo; Marco, María Pilar; Eritja Casadellà, Ramón; Poch, Marta; Martínez, Elena; Samitier, Josep
KeywordsAnti-doping
DNA chip
DNA-directed immobilization (DDI)
Fluorescence
Hapten microarray
Food safety
Immunochemical screening
Steroid
Proof of concept
Small organic molecule
Bioassay
drug determination
oligonucleotide
Issue DateJun-2009
PublisherElsevier
CitationTRAC - Trends in Analytical Chemistry
AbstractWe report a new strategy for immunochemical screening of small organic molecules based on the use of a hapten microarray. Using DNA-directed immobilization strategies, we have been able to convert a DNA chip into a hapten microarray by taking advantage of all the benefits of the structural and electrostatic homogeneous properties of DNA. The hapten microarray uses hapten-oligonucleotide probes instead of proteins, avoiding the limitations of preparing stochiometrically-defined protein-oligonucleotide bioconjugates. As proof of concept, we show here the development of a microarray for analysis of anabolic androgenic steroids. The microchip is able to detect several illegal substances with sufficient detectability to be used as a screening method, according to the regulations of the World Anti-Doping Agency for sport and the European Commision for food safety. The results that we show corroborate the universal possibilities of the DNA chip, and, in this case, they open the way to develop hapten microarrays for the immunochemical analysis of small organic molecules.
Publisher version (URL)DOI: 10.1016/j.trac.2009.04.003
URIhttp://hdl.handle.net/10261/125056
DOI10.1016/j.trac.2009.04.003
Appears in Collections:(IQAC) Artículos
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