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dc.contributor.authorGrimau, Marta G.es_ES
dc.contributor.authorEritja Casadellà, Ramónes_ES
dc.contributor.authorAviñó, Annaes_ES
dc.contributor.authorGargallo, Raimundoes_ES
dc.date.accessioned2015-11-12T08:09:32Z-
dc.date.available2015-11-12T08:09:32Z-
dc.date.issued2005-
dc.identifier.citationChemistry and Biodiversityes_ES
dc.identifier.urihttp://hdl.handle.net/10261/124869-
dc.description.abstractCyclic oligonucleotides carrying an oligopurine Watson - Crick sequence linked to the corresponding (G,A)-and (G,T)-antiparallel strands were prepared by nonenzymatic template-assisted cyclization of phosphorylated precursors. Cyclization was attempted using 3′-phosphate and 5′-phosphate linear precursors with carbodiimide or BrCN activation. The best results were obtained with the 5′-phosphorylated precursors and carbodiimide activation. Cyclic oligonucleotides bind polypyrimidine target sequence by formation of antiparallel triplexes. We have used UV and circular dichroism (CD) spectroscopy to analyze triplexes formed by cyclic oligonucleotides carrying G and A in the reverse-Hoogsteen strand. The relative stability of the triplexes formed by cyclic and linear oligonucleotides with a common polypyrimidine target was determined by melting experiments. The most-stable triplexes were formed by the cyclic oligonucleotide, followed by the unphosphorylated and phosphorylated oligonucleotide precursors, and, finally, the corresponding hairpin. Although the differences in binding affinity between cyclic oligonucleotides and their corresponding linear precursors are small, the use of cyclic oligonucleotides offers a clear advantage over conventional duplex recognition.es_ES
dc.language.isoenges_ES
dc.publisherJohn Wiley & Sonses_ES
dc.relation.isversionofPostprintes_ES
dc.rightsopenAccesses_ES
dc.subjectprotein precursores_ES
dc.subjectCircular Dichroismes_ES
dc.subjectoligonucleotideses_ES
dc.subjectNucleotides, Cyclices_ES
dc.subjectNucleic Acid Conformationes_ES
dc.subjectpurine derivativees_ES
dc.subjectsequence analysises_ES
dc.subjectgene sequencees_ES
dc.titleSynthesis and triplex-forming properties of cyclic oligonucleotides with (G,A)-antiparallel strandses_ES
dc.typeartículoes_ES
dc.identifier.doi10.1002/cbdv.200590010-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionDOI: 10.1002/cbdv.200590010es_ES
dc.rights.licensehttp://www.sherpa.ac.uk/romeo/issn/1612-1872/es_ES
dc.relation.csices_ES
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