English
español
Please use this identifier to cite or link to this item:
http://hdl.handle.net/10261/124863
Share/Impact:
Statistics |
![]() ![]() ![]() |
|
|
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |||
|
Title: | Hybridization and melting behavior of Peptide Nucleic Acid (PNA) oligonucleotide chimeras conjugated to gold nanoparticles |
Authors: | Murphy, Deirdre Brendan; Eritja Casadellà, Ramón ![]() |
Keywords: | DNA Gold Nanostructured materials Organic polymers Nucleic acids Glutamic acid Olegonucleotides |
Issue Date: | 2004 |
Publisher: | John Wiley & Sons |
Citation: | Helvetica Chimica Acta |
Abstract: | Peptide nucleic acids (PNA) and PNA-DNA chimeras carrying thiol groups were used for surface functionalization of Au nanoparticles. Conjugation of PNA to citrate-stabilized Au nanoparticles destabilized the nanoparticles causing them to precipitate. Addition of a tail of glutamic acid to the PNA prevented destabilization of the nanoparticles but resulted in loss of interaction with complementary sequences. Importantly, PNA-DNA chimeras gave stable conjugates with Au nanoparticles. The hybridization and melting properties of complexes formed from chimera-nanoparticle conjugates and oligonucleotide-nanoparticle conjugates are described for the first time. Similar to oligonucleotide- nanoparticle conjugates, conjugates with PNA-DNA chimeras gave sharper and more-defined melting profiles than those obtained with unmodified oligonucleotides. In addition, mismatch discrimination was found to be more efficient than with unmodified oligonucleotides. |
Publisher version (URL): | DOI: 10.1002/hlca.200490245 |
URI: | http://hdl.handle.net/10261/124863 |
DOI: | http://dx.doi.org/10.1002/hlca.200490245 |
Appears in Collections: | (IQAC) Artículos |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
HCA2004Murphy.pdf | Artículo principal | 93,53 kB | Adobe PDF | ![]() View/Open |
Show full item record
Review this work
Review this work
WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.