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Título: | Hybridization and melting behavior of Peptide Nucleic Acid (PNA) oligonucleotide chimeras conjugated to gold nanoparticles |
Autor: | Murphy, Deirdre Brendan; Eritja Casadellà, Ramón CSIC ORCID ; Redmond, Gareth; García de la Torre, Beatriz CSIC ORCID | Palabras clave: | DNA Gold Nanostructured materials Organic polymers Nucleic acids Glutamic acid Olegonucleotides |
Fecha de publicación: | 2004 | Editor: | John Wiley & Sons | Citación: | Helvetica Chimica Acta | Resumen: | Peptide nucleic acids (PNA) and PNA-DNA chimeras carrying thiol groups were used for surface functionalization of Au nanoparticles. Conjugation of PNA to citrate-stabilized Au nanoparticles destabilized the nanoparticles causing them to precipitate. Addition of a tail of glutamic acid to the PNA prevented destabilization of the nanoparticles but resulted in loss of interaction with complementary sequences. Importantly, PNA-DNA chimeras gave stable conjugates with Au nanoparticles. The hybridization and melting properties of complexes formed from chimera-nanoparticle conjugates and oligonucleotide-nanoparticle conjugates are described for the first time. Similar to oligonucleotide- nanoparticle conjugates, conjugates with PNA-DNA chimeras gave sharper and more-defined melting profiles than those obtained with unmodified oligonucleotides. In addition, mismatch discrimination was found to be more efficient than with unmodified oligonucleotides. | Versión del editor: | DOI: 10.1002/hlca.200490245 | URI: | http://hdl.handle.net/10261/124863 | DOI: | 10.1002/hlca.200490245 |
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HCA2004Murphy.pdf | Artículo principal | 93,53 kB | Adobe PDF | Visualizar/Abrir |
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