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The pH stability of foot-and-mouth disease virus particles is modulated by residues located at the pentameric interface and in the N terminus of VP1

AutorCaridi, Flavia ; Vázquez-Calvo, Ángela ; Sobrino Castelló, Francisco ; Martín-Acebes, Miguel Ángel
Fecha de publicaciónmay-2015
EditorAmerican Society for Microbiology
CitaciónJournal of Virology 89(10): 5633-5642 (2015)
ResumenThe picornavirus foot-and-mouth disease virus (FMDV) is the etiological agent of a highly contagious disease that affects important livestock species. The FMDV capsid is highly acid labile, and viral particles lose infectivity due to their disassembly at pH values slightly below neutrality. This acid sensitivity is related to the mechanism of viral uncoating and genome penetration from endosomes. In this study, we have analyzed the molecular basis of FMDV acid-induced disassembly by isolating and characterizing a panel of novel FMDV mutants differing in acid sensitivity. Amino acid replacements altering virion stability were preferentially distributed in two different regions of the capsid: the N terminus of VP1 and the pentameric interface. Even more, the acid labile phenotype induced by a mutation located at the pentameric interface in VP3 could be compensated by introduction of an amino acid substitution in the N terminus of VP1. These results indicate that the acid sensitivity of FMDV can be considered a multifactorial trait and that virion stability is the fine-tuned product of the interaction between residues from different capsid proteins, in particular those located within the N terminus of VP1 or close to the pentameric interface.
URIhttp://hdl.handle.net/10261/124822
DOI10.1128/JVI.03358-14
Identificadoresdoi: 10.1128/JVI.03358-14
issn: 1098-5514
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