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Profiling of different bioactive compounds in functional drinks by high-performance liquid chromatographystar

AutorMendiola León, Jose Antonio ; Marin, Francisco R. ; Señorans, F. Javier; Reglero, Guillermo ; Martín-Álvarez, Pedro J. ; Cifuentes, Alejandro ; Ibáñez, Elena
Palabras claveFunctional foods
Water-soluble vitamins
Fat-soluble vitamins
Phenolic compounds
Antioxidant activity
Fecha de publicación2008
CitaciónAdvertisement Journal of Chromatography A Volume 1188, Issue 2, 25 April 2008, Pages 234-241
ResumenIn the present work, an HPLC method is proposed to simultaneously detect and quantify water- and fat-soluble vitamins, phenolic compounds, carotenoids and chlorophylls in a single run, by using an ultradeactivated C18 column and gradient separation using trifluoroacetic acid, water and methanol. It is shown that the HPLC method provides baseline separation of all these compounds with good resolution values in 40 min. Moreover, other figures of merit of the method show a good linear response and low detection limits for all the compounds considered in the present study. Furthermore, the usefulness of this method is demonstrated via its successful application to the analysis of different beverages from different natural origin (orange, strawberry, apple, peach pineapple, plum and blackcurrant juices, soybean milk, beers) without the need of any previous sample preparation. A good correlation is also found by comparing the total phenol content (measured by Folin–Ciocalteu method) with the sum of total phenolic compounds obtained using the proposed HPLC method. By using statistical tools, the main compounds associated with antioxidant activity of the extracts (measured by 1,1-diphenyl-2-picrylhydrazyl radical scavenging) were assessed.
Versión del editorhttp://dx.doi.org/10.1016/j.chroma.2008.02.054
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