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Comparison of biological and chemical methods, and internal and external standards, for assaying tannins in Spanish shrub species

AuthorsÁlvarez del Pino, María C. ; Hervás, Gonzalo ; Mantecón, Ángel R. ; Giráldez, Francisco Javier
KeywordsBiological tannin assay
Chemical tannin assay
Internal standard
In vitro gas production
Quebracho tannins
Tannic acid
Issue Date2005
PublisherJohn Wiley & Sons
CitationJournal of the Science of Food and Agriculture 85(4): 583-590 (2005)
AbstractTannin contents of stems, flowers and leaves of four shrub species (Erica arborea, Erica australis, Cytisus cantabricus and Genista occidentalis) were chemically assessed using two colorimetric assays (butanol-HCl and Folin-Ciocalteu) and several standards (tannic acid, purified quebracho condensed tannins (CT) and purified CT extracted from each species). A method based on the biological activity of tannins in the rumen was also used for assaying tannins. Depending on the species, the use of quebracho tannin as standard over- or underestimated CT contents, in comparison to the use of the respective internal standard. However, the high correlationship observed between in vitro gas production in the presence or absence of polyethylene glycol (PEG) and CT contents estimated from quebracho suggests that this standard could be used for a quicker estimate of the nutritive value of tannin-containing plants. Tannin contents obtained by the Folin-Ciocalteu method, using tannic acid as standard, were also related to their activity on rumen fermentation. The use of the in vitro gas production technique, in the absence or presence of PEG, may provide more useful information than the chemical assays when the final aim of tannin analysis is to assess the nutritive value of feedstuffs for ruminants.
Description8 pages, 1 figure, 4 tables.-- Available online 26 Nov 2004.
Previously published as proceeding at the XXXIII Jornadas de Estudio AIDA (IX Jornadas sobre Producción animal). ITEA vol. Extra 22 (Zaragoza, Spain, May 25-27, 2001). https://digital.csic.es/handle/10262/14433
Previously published as proceeding at the XXXV Jornadas de Estudio AIDA (X Jornadas sobre Producción animal). ITEA Vol. Extra 24 (Zaragoza, Spain, May 14-16, 2003). https://digital.csic.es/handle/10261/13813
Publisher version (URL)http://dx.doi.org/10.1002/jsfa.1997
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