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Title

Synthesis of branched oligonucleotides as templates for the assembly of nanomaterials

AuthorsGrimau, Marta G.; Iacopino, Daniela; Aviñó, Anna ; García De la Torre, Beatriz; Eritja Casadellà, Ramón ; Fitzmaurice, Donald J.; Ongaro, Andrea
KeywordsMass spectrometry
Proteins
Transmission electron microscopy
Biotin
DNA hybridization
transmission electron microscopy
Issue Date2003
PublisherJohn Wiley & Sons
CitationHelvetica Chimica Acta
AbstractBranched oligonucleotides with symmetric arms 6-15, which may contain biotin as a second recognition code, were prepared. These molecules are designed to be used for the directed assembly of nanomaterials. The branched structure of the desired oligonucleotides was confirmed by mass spectrometry on small branched oliognucleotides, by gel electrophoresis, and by hybridization with complementary oligonucleotide - nanoparticle conjugates, followed by visualization of the complexes by transmission electron microscopy.
Publisher version (URL)DOI: 10.1002/hlca.200390232
URIhttp://hdl.handle.net/10261/124758
DOIhttp://dx.doi.org/10.1002/hlca.200390232
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