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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Bernal-Cabas, Margarita | - |
dc.contributor.author | Ayala, Juan Alfonso | - |
dc.contributor.author | Raivio, Tracy L. | - |
dc.date.accessioned | 2015-11-10T11:16:24Z | - |
dc.date.available | 2015-11-10T11:16:24Z | - |
dc.date.issued | 2015-02 | - |
dc.identifier | doi: 10.1128/JB.02449-14 | - |
dc.identifier | issn: 1098-5530 | - |
dc.identifier.citation | Journal of Bacteriology 197(3): 603-614 (2015) | - |
dc.identifier.uri | http://hdl.handle.net/10261/124740 | - |
dc.description.abstract | The Cpx envelope stress response mediates a complex adaptation to conditions that cause protein misfolding in the periplasm. A recent microarray study demonstrated that Cpx response activation led to changes in the expression of genes known, or predicted, to be involved in cell wall remodeling. We sought to characterize the changes that the cell wall undergoes during activation of the Cpx pathway in Escherichia coli. Luminescent reporters of gene expression confirmed that LdtD, a putative L,D-transpeptidase; YgaU, a protein of unknown function; and Slt, a lytic transglycosylase, are upregulated in response to Cpx-inducing conditions. Phosphorylated CpxR binds to the upstream regions of these genes, which contain putative CpxR binding sites, suggesting that regulation is direct. We show that the activation of the Cpx response causes an increase in the abundance of diaminopimelic acid (DAP)-DAP cross-links that involves LdtD and YgaU. Altogether, our data indicate that changes in peptidoglycan structure are part of the Cpx-mediated adaptation to envelope stress and indicate a role for the uncharacterized gene ygaU in regulating cross-linking. | - |
dc.description.sponsorship | This work was supported by Canadian Institute of Health Research operating grant 97819 and Natural Science and Engineering Research Council grant RGPIN 238422-2013. T.L.R. was supported by a senior scholar award from Alberta Innovates Health Solutions. We acknowledge financial support (project number BFU2009-09200) from the Spanish Ministry of Economy and Competitiveness to J.A.A. | - |
dc.publisher | American Society for Microbiology | - |
dc.relation.isversionof | Publisher's version | - |
dc.rights | openAccess | - |
dc.title | The Cpx envelope stress response modifies peptidoglycan cross-linking via the L,D-transpeptidase LdtD and the novel protein YgaU | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1128/JB.02449-14 | - |
dc.date.updated | 2015-11-10T11:16:25Z | - |
dc.description.version | Peer Reviewed | - |
dc.language.rfc3066 | eng | - |
dc.contributor.funder | Canadian Institutes of Health Research | - |
dc.contributor.funder | Natural Sciences and Engineering Research Council of Canada | - |
dc.contributor.funder | Alberta Innovates Health Solutions | - |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000024 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000038 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000145 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.identifier.pmid | 25422305 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
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Ayala JA The Cpx.pdf | 664 kB | Adobe PDF | Visualizar/Abrir |
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