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Título

Formation of triple-stranded DNA at d(GA·TC)(n) sequences prevents nucleosome assembly and is hindered by nucleosomes

AutorEspinàs, Maria Lluïsa ; Jiménez-García, Emilio; Martínez-Balbás, Marian ; Azorín, Ferran
Fecha de publicación1996
EditorAmerican Society for Biochemistry and Molecular Biology
CitaciónJournal of Biological Chemistry 271(50): 31807-31812 (1996)
ResumenSimple repeating d(GA·TC)(n) DNA sequences are frequently found at eukaryotic promoters, and in some cases they have been shown to be nucleosome free in vivo. These sequences show a high degree of structural polymorphism and are capable of adopting several types of non-B-DNA conformations. Here we show that the structural versatility of these sequences affects their ability to be packed into nucleosomes. Nucleosome assembly onto short double-stranded DNA fragments carrying d(GA·TC)(n) sequences of different length (n = 10 and n = 22) is very efficient. However, when the simple repeating sequence is forming a [CT(GA·TC)] triplex, nucleosome assembly is either prevented, as in the case of the d(GA·TC)22 sequence, or results in the destabilization of the triple-stranded conformation, as in the case of the d(GA·TC)10 sequence. Similarly, formation of triple-stranded DNA is hindered when the sequence is organized as nucleosomes. Efficient formation of triplex DNA occurs only at relatively high ionic strength (0.6 M NaCl), when the nucleosome is partially destabilized, and results in the disruption of the nucleosomal particle. These results indicate that nucleosome assembly and triplex formation are competing processes
Versión del editorhttp://dx.doi.org/10.1074/jbc.271.50.31807
URIhttp://hdl.handle.net/10261/124661
DOI10.1074/jbc.271.50.31807
ISSN0021-9258
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