Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/124623
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Quantitative analysis of proteins of metabolism by reverse phase protein microarrays identifies potential biomarkers of rare neuromuscular diseases |
Autor: | Santacatterina, Fulvio CSIC ORCID; Chamorro, Margarita CSIC; Núñez de Arenas, Cristina CSIC ORCID; Navarro, Carmen CSIC; Martín, Miguel Ángel; Cuezva, José M. CSIC ORCID; Sánchez-Aragó, María CSIC | Fecha de publicación: | 18-feb-2015 | Editor: | BioMed Central | Citación: | Journal of Translational Medicine 13: 65 (2015) | Resumen: | Muscle diseases have been associated with changes in the expression of proteins involved in energy metabolism. To this aim we have developed a number of monoclonal antibodies against proteins of energy metabolism. Methods: Herein, we have used Reverse Phase Protein Microarrays (RPMA), a high throughput technique, to investigate quantitative changes in protein expression with the aim of identifying potential biomarkers in rare neuromuscular diseases. A cohort of 73 muscle biopsies that included samples from patients diagnosed of Duchenne (DMD), Becker (BMD), symptomatic forms of DMD and BMD in female carriers (Xp21 Carriers), Limb Girdle Muscular Dystrophy Type 2C (LGMD2C), neuronal ceroid lipofuscinosis (NCL), glycogenosis type V (Mc Ardle disease), isolated mitochondrial complex I deficiency, intensive care unit myopathy and control donors were investigated. The nineteen proteins of energy metabolism studied included members of the mitochondrial oxidation of pyruvate, the tricarboxylic acid cycle, ß-oxidation of fatty acids, electron transport and oxidative phosphorylation, glycogen metabolism, glycolysis and oxidative stress using highly specific antibodies. Results: The results indicate that the phenotype of energy metabolism offers potential biomarkers that could be implemented to refine the understanding of the biological principles of rare diseases and, eventually, the management of these patients. Conclusions: We suggest that some biomarkers of energy metabolism could be translated into the clinics to contribute to the improvement of the clinical handling of patients affected by rare diseases. | Versión del editor: | http://dx.doi.org/10.1186/s12967-015-0424-1 | URI: | http://hdl.handle.net/10261/124623 | DOI: | 10.1186/s12967-015-0424-1 | Identificadores: | issn: 1479-5876 |
Aparece en las colecciones: | (CBM) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Cuezva JM Quantitative.pdf | 1,32 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
13
checked on 17-abr-2024
SCOPUSTM
Citations
21
checked on 15-abr-2024
WEB OF SCIENCETM
Citations
21
checked on 27-feb-2024
Page view(s)
336
checked on 18-abr-2024
Download(s)
252
checked on 18-abr-2024