English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/124552
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Tackling proteome changes in the longissimus thoracis bovine muscle in response to pre-slaughter stress

AutorFranco, Daniel ; Mato, Ariadna; Salgado, Francisco J.; López-Pedrouso, María; Carrera, Mónica ; Bravo, Susana; Parrado, María; Gallardo, José Manuel ; Zapata, Carlos
Palabras clavePre-slaughter stress biomarkers
Bos taurus
DFD meat
Longissimus thoracis
Proteome
Relative change measure
Fecha de publicación2015
EditorElsevier
CitaciónJournal of Proteomics 122: 73-85 (2015)
ResumenPre-slaughter stress has adverse effects on meat quality that can lead to the occurrence of Dark Firm Dry (DFD) meat in cattle. This study explores the previously uncharacterized proteome changes linked to pre-slaughter stress in the longissimus thoracis (LT) bovine muscle. Differential proteome profiles of DFD and normal (non-DFD) LT meat samples from male calves of the Rubia Gallega breed were assessed by 2-DE coupled to MS analysis (LC-MS/MS and MALDI TOF/TOF MS). A total of seven structural-contractile proteins (three different myosin light chain isoforms, two fast skeletal myosin light chain 2 isoforms, troponin C type 2 and cofilin-2) and three metabolism enzymes (triosephosphate isomerase, ATP synthase and beta-galactoside alpha-2,6-sialyltransferase) were found to have statistically significant differential abundance in sample groups. In addition, 2-DE in combination with the phosphoprotein-specific fluorescent dye Pro-Q DPS revealed that highly phosphorylated fast skeletal myosin regulatory light chain 2 isoforms underwent the most intense relative change in muscle conversion to DFD meat. Therefore, they appear to be the most sensitive biomarkers of stress just prior to slaughter in Rubia Gallega. Overall, these findings will facilitate a more integrative understanding of the biochemical processes associated with stress in cattle muscle and their effects in meat quality
Descripción13 páginas, 5 figuras, 4 tablas
Versión del editorhttp://dx.doi.org/10.1016/j.jprot.2015.03.029
URIhttp://hdl.handle.net/10261/124552
DOI10.1016/j.jprot.2015.03.029
ISSN1874-3919
E-ISSN1876-7737
Aparece en las colecciones: (IIM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Tacling_proteome_changes_2015.pdf372,33 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.