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GC-FID determination and pharmacokinetic studies of oleanolic acid in human serum

AuthorsRada, Mirella ; Castellano, José María ; Perona, Javier S. ; Guinda Garín, Mª Ángeles
Issue Date2015
PublisherJohn Wiley & Sons
CitationBiomedical Chromatography 29: 1687- 1692 (2015)
Abstract© 2015 John Wiley & Sons, Ltd. Analytical interest of OA determination in human serum has increased owing to the increasing interest in pharmaceutical research by pharmaceutical properties. A simple, specific, precise and accurate GC method with flame ionization detector (FID) developed and validated for the determination of oleanolic acid (OA) in human serum (HS). To an aliquot of HS, internal standard was added and a combination of liquid-liquid extraction with a mixture of diethyl ether-isopropyl alcohol, filtration and consecutive GC resulted in separation and quantification of OA. The organic phase was analyzed using a GC system equipped with a 30×0.25mm i.d. Rtx-65TG capillary column and FID detection. Total chromatographic time was 10min and no interfering peaks from endogenous components in blank serum were observed. The OA/internal standard peak area ratio was linearly fitted to the OA concentration (r=0.992) over the range 10-1500ng/mL. The mean serum extraction recovery of OA was 96.7±1.0% and the lower limit of quantification based on 5mL of serum was 10.7ng/mL. The intra-day coefficient of variation ranged from 1.3 to 3.6% and inter-day varied from 1.4 to 4.5%. The developed method was used to study the pharmacokinetics of OA after oral administration in humans. The assay was simple, sensitive, precise and accurate for the use in the study of the mechanisms of absorption and distribution of OA in humans.
Identifiersdoi: 10.1002/bmc.3480
issn: 1099-0801
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