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Polymorphic crystal structures of an all-AT DNA dodecamer

AutorAcosta-Reyes, F. J..; Subirana, Juan A.; Pous, Joan; Sánchez-Giraldo, Raquel; Condom, Núria; Baldini, Roberto; Malinina, Lucy; Campos, J. Lourdes
Palabras claveDNA
AT-rich DNA
X-ray crystallography
inorganic cations
liquid crystals
crossed DNA structures
Fecha de publicación23-dic-2014
EditorJohn Wiley & Sons
CitaciónBiopolymers 103(3): 123-133 (2014)
Resumen© 2014 Wiley Periodicals, Inc. Biopolymers 103: 123-133, 2015. In this work, we explore the influence of different solvents and ions on the crystallization behavior of an all-AT dodecamer d(AATAAATTTATT)2 In all cases, the oligo-nucleotides are found as continuous columns of stacked duplexes. The spatial organization of such columns is variable; consequently we have obtained seven different crystal forms. The duplexes can be made to crystallize in either parallel or crossed columns. Such versatility in the formation of a variety of crystal forms is characteristic for this sequence. It had not been previously reported for any other sequence. In all cases, the oligonucleotide duplexes have been found to crystallize in the B form. The crystallization conditions determine the organization of the crystal, although no clear local interactions have been detected. Mg2+ and Ni2+ can be used in order to obtain compact crossed structures. DNA-DNA interactions in the crystals of our all-AT duplexes present crossovers which are different from those previously reported for mixed sequence oligonucleotides. Our results demonstrate that changes in the ionic atmosphere and the crystallization solvent have a strong influence on the DNA-DNA interactions. Similar ionic changes will certainly influence the biological activity of DNA. Modulation of the crystal structure by ions should also be explored in DNA crystal engineering. Liquid crystals with a peculiar macroscopic shape have also been observed.
Versión del editorhttp://dx.doi.org/10.1002/bip.22565
Identificadoresdoi: 10.1002/bip.22565
issn: 1097-0282
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