Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/124254
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Fascin links Btl/FGFR signalling to the actin cytoskeleton during Drosophila tracheal morphogenesis |
Autor: | Okenve-Ramos, Pilar CSIC ORCID; Llimargas, Marta CSIC ORCID | Palabras clave: | Actin cytoskeleton Cell migration Drosophila tracheal system Fascin FGFR pathway Morphogenesis |
Fecha de publicación: | 4-feb-2014 | Editor: | Company of Biologists | Citación: | Development 141(4): 929-939 (2014) | Resumen: | A key challenge in normal development and in disease is to elucidate the mechanisms of cell migration. Here we approach this question using the tracheal system of Drosophila as a model. Tracheal cell migration requires the Breathless/FGFR pathway; however, how the pathway induces migration remains poorly understood. We find that the Breathless pathway upregulates singed at the tip of tracheal branches, and that this regulation is functionally relevant. singed encodes Drosophila Fascin, which belongs to a conserved family of actin-bundling proteins involved in cancer progression and metastasis upon misregulation. We show that singed is required for filopodia stiffness and proper morphology of tracheal tip cells, defects that correlate with an abnormal actin organisation. We propose that singed-regulated filopodia and cell fronts are required for timely and guided branch migration and for terminal branching and branch fusion. We find that singed requirements rely on its actin-bundling activity controlled by phosphorylation, and that active Singed can promote tip cell features. Furthermore, we find that singed acts in concert with forked, another actin cross-linker. The absence of both cross-linkers further stresses the relevance of tip cell morphology and filopodia for tracheal development. In summary, our results on the one hand reveal a previously undescribed role for forked in the organisation of transient actin structures such as filopodia, and on the other hand identify singed as a new target of Breathless signal, establishing a link between guidance cues, the actin cytoskeleton and tracheal morphogenesis. © 2014. Published by The Company of Biologists Ltd. | Versión del editor: | http://dx.doi.org/10.1242/dev.103218 | URI: | http://hdl.handle.net/10261/124254 | DOI: | 10.1242/dev.103218 | Identificadores: | doi: 10.1242/dev.103218 issn: 0950-1991 |
Aparece en las colecciones: | (IBMB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Okenve-Development-2015-v141-929.pdf | 6,63 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
26
checked on 15-mar-2024
WEB OF SCIENCETM
Citations
24
checked on 25-feb-2024
Page view(s)
317
checked on 19-mar-2024
Download(s)
303
checked on 19-mar-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.