English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/124254
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Fascin links Btl/FGFR signalling to the actin cytoskeleton during Drosophila tracheal morphogenesis

AuthorsOkenve-Ramos, Pilar; Llimargas, Marta
KeywordsActin cytoskeleton
Cell migration
Drosophila tracheal system
Fascin
FGFR pathway
Morphogenesis
Issue Date4-Feb-2014
PublisherCompany of Biologists
CitationDevelopment 141(4): 929-939 (2014)
AbstractA key challenge in normal development and in disease is to elucidate the mechanisms of cell migration. Here we approach this question using the tracheal system of Drosophila as a model. Tracheal cell migration requires the Breathless/FGFR pathway; however, how the pathway induces migration remains poorly understood. We find that the Breathless pathway upregulates singed at the tip of tracheal branches, and that this regulation is functionally relevant. singed encodes Drosophila Fascin, which belongs to a conserved family of actin-bundling proteins involved in cancer progression and metastasis upon misregulation. We show that singed is required for filopodia stiffness and proper morphology of tracheal tip cells, defects that correlate with an abnormal actin organisation. We propose that singed-regulated filopodia and cell fronts are required for timely and guided branch migration and for terminal branching and branch fusion. We find that singed requirements rely on its actin-bundling activity controlled by phosphorylation, and that active Singed can promote tip cell features. Furthermore, we find that singed acts in concert with forked, another actin cross-linker. The absence of both cross-linkers further stresses the relevance of tip cell morphology and filopodia for tracheal development. In summary, our results on the one hand reveal a previously undescribed role for forked in the organisation of transient actin structures such as filopodia, and on the other hand identify singed as a new target of Breathless signal, establishing a link between guidance cues, the actin cytoskeleton and tracheal morphogenesis. © 2014. Published by The Company of Biologists Ltd.
Publisher version (URL)http://dx.doi.org/10.1242/dev.103218
URIhttp://hdl.handle.net/10261/124254
DOI10.1242/dev.103218
Identifiersdoi: 10.1242/dev.103218
issn: 0950-1991
Appears in Collections:(IBMB) Artículos
Files in This Item:
File Description SizeFormat 
Okenve-Development-2015-v141-929.pdf6,63 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.