English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/124231
Título

Bioactive membranes for bone regeneration applications: Effect of physical and biomolecular signals on mesenchymal stem cell behavior

AutorTejeda-Montes, Esther; Smith, Katherine H.; Rebollo, Elena ; Gómez, Raúl; Alonso, Matilde; Rodríguez-Cabello, José Carlos; Engel, Elisabeth; Mata, Álvaro
Palabras claveBioactivity
Elastin-like recombinamers
Membranes
Periosteal grafts
Surface topographies
Fecha de publicaciónene-2014
EditorElsevier
CitaciónActa Biomaterialia 10(1): 134-141 (2014)
ResumenThis study focuses on the in vitro characterization of bioactive elastin-like recombinamer (ELR) membranes for bone regeneration applications. Four bioactive ELRs exhibiting epitopes designed to promote mesenchymal stem cell adhesion (RGDS), endothelial cell adhesion (REDV), mineralization (HAP), and both cell adhesion and mineralization (HAP-RGDS) were synthesized using standard recombinant protein techniques. The materials were then used to fabricate ELR membranes incorporating a variety of topographical micropatterns including channels, holes and posts. Primary rat mesenchymal stem cells (rMSCs) were cultured on the different membranes and the effects of biomolecular and physical signals on cell adhesion, morphology, proliferation, and differentiation were evaluated. All results were analyzed using a custom-made MATLAB program for high throughput image analysis. Effects on cell morphology were mostly dependent on surface topography, while cell proliferation and cell differentiation were largely dependent on the biomolecular signaling from the ELR membranes. In particular, osteogenic differentiation (evaluated by staining for the osteoblastic marker osterix) was significantly enhanced on cells cultured on HAP membranes. Remarkably, cells growing on membranes containing the HAP sequence in non-osteogenic differentiation media exhibited significant up-regulation of the osteogenic marker as early as day 5, while those growing on fibronectin-coated glass in osteogenic differentiation media did not. These results are part of our ongoing effort to develop an optimized molecularly designed periosteal graft. © 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
Versión del editorhttp://dx.doi.org/10.1016/j.actbio.2013.09.001
URIhttp://hdl.handle.net/10261/124231
DOI10.1016/j.actbio.2013.09.001
Identificadoresdoi: 10.1016/j.actbio.2013.09.001
issn: 1742-7061
Aparece en las colecciones: (IBMB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.