English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/124209
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Skeletal muscle myogenesis is regulated by G protein-coupled receptor kinase 2

AutorGarcía-Guerra, Lucía; Vila-Bedmar, Rocío ; Carrasco-Rando, Marta ; Cruces-Sande, Marta ; Martín-Martínez, Mercedes ; Ruiz-Gómez, Ana ; Ruiz-Gómez, Mar ; Lorenzo, Margarita; Fernández-Veledo, Sonia; Mayor Menéndez, Federico ; Murga, Cristina ; Nieto-Vázquez, Iria
Fecha de publicación2014
EditorOxford University Press
CitaciónJournal of Molecular Cell Biology 6(4): 299-311 (2014)
ResumenG protein-coupled receptor kinase 2 (GRK2) is an important serine/threonine-kinase regulating different membrane receptors and intracellular proteins. Attenuation of Drosophila Gprk2 in embryos or adult flies induced a defective differentiation of somatic muscles, loss of fibers, and a flightless phenotype. In vertebrates, GRK2 hemizygous mice contained less but more hypertrophied skeletal muscle fibers than wild-type littermates. In C2C12 myoblasts, overexpression of a GRK2 kinase-deficient mutant (K220R) caused precocious differentiation of cells into immature myotubes, which were wider in size and contained more fused nuclei, while GRK2 overexpression blunted differentiation. Moreover, p38MAPK and Akt pathways were activated at an earlier stage and to a greater extent in K220R-expressing cells or upon kinase downregulation, while the activation of both kinases was impaired in GRK2-overexpressing cells. The impaired differentiation and fewer fusion events promoted by enhanced GRK2 levels were recapitulated by a p38MAPK mutant, which was able to mimic the inhibitory phosphorylation of p38MAPK by GRK2, whereas the blunted differentiation observed in GRK2-expressing clones was rescued in the presence of a constitutively active upstream stimulator of the p38MAPK pathway. These results suggest that balanced GRK2 function is necessary for a timely and complete myogenic process.
URIhttp://hdl.handle.net/10261/124209
DOI10.1093/jmcb/mju025
Identificadoresdoi: 10.1093/jmcb/mju025
issn: 1674-2788
e-issn: 1759-4685
Aparece en las colecciones: (CBM) Artículos
(IIBM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.