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Title

A third subunit in ancestral cytochrome c-dependent nitric oxide reductases

AuthorsBricio, Carlos; Álvarez, Laura ; San Martín, Marta; Schurig Briccio, Lici A.; Gennis, Robert B.; Berenguer, José
Issue Date6-Jun-2014
PublisherAmerican Society for Microbiology
CitationApplied and Environmental Microbiology 80: 4871- 4878 (2014)
AbstractReduction of NO to N2O by denitrifiying bacteria is catalyzed either by a monomeric quinol-nitric oxide reductase (qNor) or by a heterodimeric cytochrome c-dependent nitric oxide reductase (cNor). In ancient thermophilic bacteria belonging to the Thermales and Aquificales phylogenetic groups, the cluster encoding the cNor includes a small third gene (norH), in addition to those encoding homologues to the subunits of a typical cNor (norC and norB). We show in Thermus thermophilus that the three genes are cotranscribed in a single mRNA from an inducible promoter. The isolation of individual nor mutants and the production in vivo of His-tagged NorH protein followed by immobilized-metal affinity chromatography (IMAC) allowed us to conclude that NorH constitutes a third subunit of the cNor from T. thermophilus, which is involved in denitrification in vivo, likely allowing more efficient electron transport to cNor. © 2014, American Society for Microbiology.
URIhttp://hdl.handle.net/10261/124109
DOI10.1128/AEM.00790-14
Identifiersdoi: 10.1128/AEM.00790-14
issn: 1098-5336
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