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Structure of the N-terminal domain of the protein Expansion: An 'Expansion' to the Smad MH2 fold

AutorBeich-Frandsen, Mads; Aragón, Eric; Llimargas, Marta ; Benach, Jordi; Riera, Antoni; Pous, Joan; Macias, Maria J.
Palabras claveExpansion protein
Smad homology domain 2
MH2 domain
Smad/FHA protein superfamily
protein-protein interaction
phenix.mr_rosetta
Fecha de publicación1-abr-2015
EditorBlackwell Publishing
CitaciónActa Crystallographica Section D: Biological Crystallography 71(4): 844-853 (2015)
ResumenGene-expression changes observed in Drosophila embryos after inducing the transcription factor Tramtrack led to the identification of the protein Expansion. Expansion contains an N-terminal domain similar in sequence to the MH2 domain characteristic of Smad proteins, which are the central mediators of the effects of the TGF-β signalling pathway. Apart from Smads and Expansion, no other type of protein belonging to the known kingdoms of life contains MH2 domains. To compare the Expansion and Smad MH2 domains, the crystal structure of the Expansion domain was determined at 1.6Å resolution, the first structure of a non-Smad MH2 domain to be characterized to date. The structure displays the main features of the canonical MH2 fold with two main differences: the addition of an α-helical region and the remodelling of a protein-interaction site that is conserved in the MH2 domain of Smads. Owing to these differences, to the new domain was referred to as Nα-MH2. Despite the presence of the Nα-MH2 domain, Expansion does not participate in TGF-β signalling; instead, it is required for other activities specific to the protostome phyla. Based on the structural similarities to the MH2 fold, it is proposed that the Nα-MH2 domain should be classified as a new member of the Smad/FHA superfamily.
DescripciónSupporting information at doi:10.1107/S1399004715001443/kw5112sup1.pdf
Versión del editorhttp://dx.doi.org/10.1107/S1399004715001443
http://dx.doi.org/10.1107/S1399004715001443/kw5112sup1.pdf
URIhttp://hdl.handle.net/10261/124106
DOI10.1107/S1399004715001443
10.1107/S1399004715001443/kw5112sup1.pdf
Identificadoresissn: 1399-0047
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