English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/124074
Título

RNA-Seq in Mytilus galloprovincialis: comparative transcriptomics and expression profiles among different tissues

AutorMoreira, Rebeca ; Pereiro, Patricia ; Canchaya, Carlos; Posada, D.; Figueras Huerta, Antonio; Novoa, Beatriz
Palabras claveMytilus galloprovincialis
Transcriptome
NGS
RNA-Seq
NOISeq
KEGG
Gene Ontology
Blast2GO
Fecha de publicación24-sep-2015
EditorBioMed Central
CitaciónBMC Genomics 16: 728 (2015)
Resumen[Background] The Mediterranean mussel (Mytilus galloprovincialis) is a cosmopolitan, cultured bivalve with worldwide commercial and ecological importance. However, there is a qualitative and quantitative lack of knowledge of the molecular mechanisms involved in the physiology and immune response of this mollusc. In order to start filling this gap, we have studied the transcriptome of mantle, muscle and gills from naïve Mediterranean mussels and hemocytes exposed to distinct stimuli. [Results] A total of 393,316 million raw RNA-Seq reads were obtained and assembled into 151,320 non-redundant transcripts with an average length of 570 bp. Only 55 % of the transcripts were shared across all tissues. Hemocyte and gill transcriptomes shared 60 % of the transcripts while mantle and muscle transcriptomes were most similar, with 77 % shared transcripts. Stimulated hemocytes showed abundant defense and immune-related proteins, in particular, an extremely high amount of antimicrobial peptides. Gills expressed many transcripts assigned to both structure and recognition of non-self patterns, while in mantle many transcripts were related to reproduction and shell formation. Moreover, this tissue presented additional and interesting hematopoietic, antifungal and sensorial functions. Finally, muscle expressed many myofibril and calcium-related proteins and was found to be unexpectedly associated with defense functions. In addition, many metabolic routes related to cancer were represented. [Conclusions] Our analyses indicate that whereas the transcriptomes of these four tissues have characteristic expression profiles in agreement with their biological structures and expected functions, tissue-specific transcriptomes reveal a complex and specialized functions
Descripción18 páginas, 8 figuras, 5 tablas.-- This article is distributed under the terms of the Creative Commons Attribution 4.0 International License
Versión del editorhttp://dx.doi.org/10.1186/s12864-015-1817-5
URIhttp://hdl.handle.net/10261/124074
DOI10.1186/s12864-015-1817-5
E-ISSN1471-2164
Aparece en las colecciones: (IIM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
RNA-Seq_mytilus_galloprovincialis.pdf3,67 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.