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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/12400
Título

Optimization of the Extraction of Antioxidants from Dunaliella salina Microalga by Pressurized Liquids

AutorHerrero, Miguel ; Jaime, Laura ; Martín-Álvarez, Pedro J. ; Cifuentes, Alejandro ; Ibáñez, Elena
Palabras clavePLE
dunaliella salina
TEAC
antioxidant compounds
optimization
Fecha de publicación2006
EditorAmerican Chemical Society
CitaciónJ. Agric. Food Chem. 2006, 54, 5597-5603
ResumenIn this work, extraction of antioxidant compounds from Dunaliella salina microalga is optimized by combining pressurized liquid extraction (PLE) and experimental design (three-level factorial design) with three different solvents (hexane, ethanol, and water). Two main factors were considered, the extraction temperature (40, 100, and 160 °C) and the extraction time (5, 17.5, and 30 min). As response variables, the extraction yield (percent dry weight/initial weight) and the antioxidant activity of the extracts (determined using the TEAC method) were used. The parameters of the model were estimated by multiple linear regression. Results showed that the extraction temperature was the factor having the strongest influence (positive) on the two response variables. The best yields were obtained with ethanol at the higher extraction temperature and time tested. Besides, although hexane extracts provided the best antioxidant activity, ethanol extracts were also very active. The chemical characterization of ethanol extracts was carried out using HPLC-DAD, and attempts have been made to correlate their chemical composition with the antioxidant activity measured. Results pointed out that the extracts contained, besides all-trans-â-carotene and isomers, several different minor carotenoids that seemed to make a contribution to the antioxidant activity of the extracts.
Versión del editorhttp://dx.doi.org/10.1021/jf060546q
URIhttp://hdl.handle.net/10261/12400
DOI10.1021/jf060546q
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