English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/123981
Título

Analysis of the effect of the mitochondrial prohibitin complex, a context-dependent modulator of longevity, on the C. elegans metabolome

AutorLourenço, Artur B.; Muñoz-Jiménez, Celia; Venegas-Calerón, Mónica ; Artal-Sanz, Marta
Palabras claveProhibitin
Insulin signalling
Mitochondria
C. elegans
Longevity
Metabolomics
Fecha de publicaciónnov-2015
EditorElsevier
CitaciónBiochimica et Biophysica Acta - Bioenergetics 1847(11): 1457-1468 (2015)
ResumenThe mitochondrial prohibitin complex, composed of two proteins, PHB-1 and PHB-2, is a context-dependent modulator of longevity. Specifically, prohibitin deficiency shortens the lifespan of otherwise wild type worms, while it dramatically extends the lifespan under compromised metabolic conditions. This extremely intriguingly phenotype has been linked to alterations in mitochondrial function and in fat metabolism. However, the true function of the mitochondrial prohibitin complex remains elusive. Here, we used gas chromatography coupled to a flame ionization detector (GC/FID) and 1H NMR spectroscopy to gain molecular insights into the effect of prohibitin depletion on the Caenorhabditis elegans metabolome. We analysed the effect of prohibitin deficiency in two different developmental stages and under two different conditions, which result in opposing longevity phenotypes, namely wild type worms and daf-2(e1370) insulin signalling deficient mutants. Prohibitin depletion was shown to alter the fatty acid (GC/FID) and 1H NMR metabolic profiles of wild type animals both at the fourth larval stage of development (L4) and at the young adult (YA) stage, while being more pronounced at the later stage. Furthermore, wild type and the diapause mutant daf-2(e1370), either expressing or not prohibitin, were clearly distinguishable based on their metabolic profiles, revealing changes in fatty acid composition, as well as in carbohydrate and amino acid metabolism. Moreover, the metabolic data indicate that daf-2(e1370) mutants are more robust than the wild type animals to changes induced by prohibitin depletion. The impact of prohibitin depletion on the C. elegans metabolome will be discussed herein in the scope of its effect on longevity.
Descripción12 páginas; 5 figuras; 4 tablas; 4 figuras.-- This article is part of a Special Issue entitled: Mitochondrial Dysfunction in Aging. Guest Editor: Aleksandra Trifunovic.-- Under a Creative Commons license.
Versión del editorhttp://dx.doi.org/10.1016/j.bbabio.2015.06.003
URIhttp://hdl.handle.net/10261/123981
DOI10.1016/j.bbabio.2015.06.003
ISSN0005-2728
Aparece en las colecciones: (CABD) Artículos
(IG) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
BBA_2015_V1847_P1457.pdfArtículo principal1,5 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.