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DDsk2 regulates H2Bub1 and RNA polymerase II pausing at dHP1c complex target genes

AutorKessler, Roman; Tisserand, Johan; Font-Burgada, Joan; Reina, Oscar; Coch, Laura; Attolini, Camille Stephan Otto; Garcia-Bassets, Ivan; Azorín, Ferran
Fecha de publicación28-abr-2015
EditorNature Publishing Group
CitaciónNature Communications 6: 7049 (2015)
Resumen© 2015 Macmillan Publishers Limited. All rights reserved. dDsk2 is a conserved extraproteasomal ubiquitin receptor that targets ubiquitylated proteins for degradation. Here we report that dDsk2 plays a nonproteolytic function in transcription regulation. dDsk2 interacts with the dHP1c complex, localizes at promoters of developmental genes and is required for transcription. Through the ubiquitin-binding domain, dDsk2 interacts with H2Bub1, a modification that occurs at dHP1c complex-binding sites. H2Bub1 is not required for binding of the complex; however, dDsk2 depletion strongly reduces H2Bub1. Co-depletion of the H2Bub1 deubiquitylase dUbp8/Nonstop suppresses this reduction and rescues expression of target genes. RNA polymerase II is strongly paused at promoters of dHP1c complex target genes and dDsk2 depletion disrupts pausing. Altogether, these results suggest that dDsk2 prevents dUbp8/Nonstop-dependent H2Bub1 deubiquitylation at promoters of dHP1c complex target genes and regulates RNA polymerase II pausing. These results expand the catalogue of nonproteolytic functions of ubiquitin receptors to the epigenetic regulation of chromatin modifications.
Versión del editorhttp://dx.doi.org/10.1038/ncomms8049
URIhttp://hdl.handle.net/10261/123907
DOI10.1038/ncomms8049
Identificadoresdoi: 10.1038/ncomms8049
issn: 2041-1723
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