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hSNF5 is involved in cytokinesis and endocytosis by DNM2 interaction

AuthorsAlfonso-Pérez, Tatiana; Domínguez-Sánchez, María S. ; García-Domínguez, Mario ; Reyes, José C.
Issue Date4-Sep-2012
Citation22nd IUBMB - 37th FEBS Congress (2012)
AbstractThe tumor suppressor protein hSNF5 is an essential component of the mammalian SWI/SNF chromatin remodeling complex. It has been reported that this protein has a masked nuclear export signal and is transiently exported to the cytoplasm upon cell infection with HIV-1. We show the existence of a permanent fraction of hSNF5 in the cytoplasm by biochemical fractionation and immunofluorescence of cultured cells. Moreover, we demonstrate that exists a constitutive transport of EGFP-hSNF5 from the nucleus to the cytoplasm by fluorescence loss in photobleaching (FLIP), indicating that hSNF5 shuttles between the nucleus and the cytoplasm. In order to elucidate hSNF5 cytoplasmic functions, we performed a yeast two-hybrid screening to identify putative hSNF5 interacting proteins. We identified Dynamin 2 (DNM2) as a putative hSNF5 interacting protein and verified this interaction by coimmunoprecipitation, immunofluorescence colocalization and pull down experiments. DNM2 is a GTPase implicated in several cellular events including cytokinesis and endocytosis. It has been shown that this protein localizes in midbodies and that Dnm2 KO cells have cytokinesis defect. We show that hSNF5 is also present in midbodies and that hSNF5 overexpression prevents cytokinesis. Moreover, we have detected the opposite effect when antibodies against hSNF5 are transfected in cells. On the other hand, it has been demonstrated that DNM2 depletion by small interfering RNA (siRNA) inhibits endocytosis. We have seen that stability of DNM2 protein decreases in hSNF5-depleted cells, strongly impairing endocytosis. Taken together, our results reveal a new role of hSNF5 in cytokinesis and endocytosis, probably by its interaction with DNM2
DescriptionPóster presentado al 22nd IUBMB & 37th FEBS Congress: From Single Molecules to Systems Biology, celebrado en Sevilla (España) del 4 al 9 de septiembre de 2012
Appears in Collections:(CABIMER) Comunicaciones congresos
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