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Título

Novel small RNAs expresión libraries uncover hsa-mir30c and hsa-mir30b as key factors in anoikis resistance

AutorMoreno-Mateos, Miguel A. ; Barragán, Verónica ; Torres, Belén ; Méndez-Vidal, Cristina ; Pintor-Toro, José Antonio
Fecha de publicación3-may-2012
CitaciónIII Annual Conference Institut de Medicina Predictiva i Personalitzada del Càncer: RNA Biology in Cancer and other Diseases (2012)
ResumenMicroRNAs (miRNAs) have been widely studied in order to elucidate their biological functions. MicroRNA microarrays or miRNA overexpression libraries generated by synthesis and cloning of individual miRNAs have been used to study their different roles. In this work, we have developed a novel methodology to express mature miRNAs and other small RNAs from a double convergent RNA polymerase III promoter. We show that the generated miRNAs function similarly to those processed from primary transcripts or pri-miRNAs. This system allowed us to produce a lentiviral library expressing the whole population of small RNAs present in a metastatic cell line. A functional screening using this library led to the identification of hsa-miR-30b and hsa-miR-30c as negative regulators of cell death induced by loss of attachment (anoikis). Importantly, we demonstrated that the acquisition of anoikis resistance via these miRNAs is achieved through down-regulation of caspase 3 expression.
DescripciónPóster presentado en la III Annual Conference del Institut de Medicina Predictiva i Personalitzada del Càncer: RNA Biology in Cancer and other Diseases, celebrada en Barcelona del 3 al 4 de mayo de 2012
URIhttp://hdl.handle.net/10261/123749
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