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Mollicutes of non-mammal marine animals.
|Autor:||Vega-Oreallana, Orestes; Poveda, José B.; Mederos-Iriarte, Lidia E.; Poveda, Carlos G.; Caballero, M. José; Betancor, Mónica B.; Rosselló-Mora, Ramón ; Ramírez, Anna S.|
|Fecha de publicación:||7-jun-2013|
|Citación:||European Mycoplasma Meeting: Mycoplasmas - A Practical Approach (2013)|
|Resumen:||Mollicutes have been found in nearly all living beings on the planet. Mycoplasma species have been reported in marine mammals (cetaceans and pinnipeds) and fish. Among marine invertebrates, Mollicutes have been identified in bryozoans, American oysters, corals and a variety of marine crustacean species like crayfish and shrimps. This work describes the methodology and the attempts to isolate mollicutes from non-mammal marine animals. One hundred and seven fish of twenty one different species, seven octopus (Octopus vulgaris), two European flying squids (Todarodes sagittatus) and four batches of jellyfish (Pelagia nocticula) were tested for the presence of mollicutes. Samples were cultured using a variation of the SP4-II medium. Originally the SP4-II medium was supplemented with 1.5% of NaCl and incubated at 18ºC anaerobically. However, after the first isolation was obtained, the growth conditions were adjusted to 3% of NaCl and aerobically incubation at 25ºC. DNA was extracted after one month of incubation and a Class Mollicutes real time PCR has been done. Mollicutes isolations were obtained from squid in three samples; two from octopus and four from jellyfish. It was not possible to get isolations from fish, although two samples gave positive to the class PCR. Isolates were analyzed biochemically and genetically (16S-23S Intergenic Spacer Region (ISR) and 16S rDNA). PCR products were sent for sequencing. Nucleotide sequences were compared using the GeneBank software. A partial 16S rDNA sequence from a jellyfish isolation was classified as Spiroplasma spp. 16S rDNA and ISR from squids and octopus isolations differs significantly from all known Mycoplasma species, but have 98-99% of similarity with a partial 16S rDNA sequence (738 bp; AY226323) detected in environmental samples (brine water, Mediterranean Sea)|
|Descripción:||Comunicación presentada en el European Mycoplasma Meeting: Mycoplasmas - A Practical Approach, celebrado los días 6 y 7 de junio de 2013 en Dubrovnik (Croacia)|
|Aparece en las colecciones:||(IMEDEA) Comunicaciones congresos|
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