English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/123725
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Manganese Redistribution by Calcium-stimulated Vesicle Trafficking Bypasses the Need for P-type ATPase Function

AutorGarcía-Rodríguez, Néstor; Manzano-Lopez, Javier; Muñoz-Bravo, Miguel; Fernández García, Elisabet ; Muñiz, Manuel; Wellinger, Ralf Erik
Fecha de publicación10-abr-2015
EditorAmerican Society for Biochemistry and Molecular Biology
CitaciónJournal of Biological Chemistry 290(15): 9335- 9347 (2015)
ResumenRegulation of intracellular ion homeostasis is essential for eukaryotic cell physiology. An example is provided by loss of ATP2C1 function, which leads to skin ulceration, improper keratinocyte adhesion, and cancer formation in Hailey-Hailey patients. The yeast ATP2C1 orthologue PMR1 codes for a Mn(2+)/Ca(2+) transporter that is crucial for cis-Golgi manganese supply. Here, we present evidence that calcium overcomes the lack of Pmr1 through vesicle trafficking-stimulated manganese delivery and requires the endoplasmic reticulum Mn(2+) transporter Spf1 and the late endosome/trans-Golgi Nramp metal transporter Smf2. Smf2 co-localizes with the putative Mn(2+) transporter Atx2, and ATX2 overexpression counteracts the beneficial impact of calcium treatment. Our findings suggest that vesicle trafficking promotes organelle-specific ion interchange and cytoplasmic metal detoxification independent of calcineurin signaling or metal transporter re-localization. Our study identifies an alternative mode for cis-Golgi manganese supply in yeast and provides new perspectives for Hailey-Hailey disease treatment.
Versión del editorhttp://dx.doi.org/10.1074/jbc.M114.616334
Identificadoresdoi: 10.1074/jbc.M114.616334
issn: 0021-9258
e-issn: 1083-351X
Aparece en las colecciones: (IBIS) Artículos
(CABIMER) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Mostrar el registro completo

Artículos relacionados:

NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.