English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/123688
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Methacrylate-endcapped caprolactone and FM19G11 provide a proper niche for spinal cord-derived neural cells

AutorValdés-Sánchez, Teresa; Rodríguez-Jiménez, Francisco Javier; García-Cruz, Dunia M.; Escobar-Ivirico, Jorge L.; Alastrue-Agudo, Ana; Erceg, Slaven; Monleón Pradas, Manuel; Moreno-Manzano, Victoria
Palabras claveBiomaterials
Ependymal stem cells
Spinal cord injury
Fecha de publicación27-mar-2013
EditorJohn Wiley & Sons
CitaciónJournal of Tissue Engineering and Regenerative Medicine 9(6): 734- 739 (2015)
ResumenSpinal cord injury (SCI) is a cause of paralysis. Although some strategies have been proposed to palliate the severity of this condition, so far no effective therapies have been found to reverse it. Recently, we have shown that acute transplantation of ependymal stem/progenitor cells (epSPCs), which are spinal cord-derived neural precursors, rescue lost neurological function after SCI in rodents. However, in a chronic scenario with axon repulsive reactive scar, cell transplantation alone is not sufficient to bridge a spinal cord lesion, therefore a combinatorial approach is necessary to fill cavities in the damaged tissue with biomaterial that supports stem cells and ensures that better neural integration and survival occur. Caprolactone 2-(methacryloyloxy) ethyl ester (CLMA) is a monomer [obtained as a result of ε-caprolactone and 2-hydroxyethyl methacrylate (HEMA) ring opening/esterification reaction], which can be processed to obtain a porous non-toxic 3D scaffold that shows good biocompatibility with epSPC cultures. epSPCs adhere to the scaffolds and maintain the ability to expand the culture through the biomaterial. However, a significant reduction of cell viability of epSPCs after 6days in vitro was detected. FM19G11, which has been shown to enhance self-renewal properties, rescues cell viability at 6days. Moreover, addition of FM19G11 enhances the survival rates of mature neurons from the dorsal root ganglia when cultured with epSPCs on 3D CLMA scaffolds. Overall, CLMA porous scaffolds constitute a good niche to support neural cells for cell transplantation approaches that, in combination with FM19G11, offer a new framework for further trials in spinal cord regeneration.
Versión del editorhttp://dx.doi.org/10.1002/term.1735
Identificadoresdoi: 10.1002/term.1735
issn: 1932-7005
Aparece en las colecciones: (CABIMER) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Mostrar el registro completo

Artículos relacionados:

NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.