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http://hdl.handle.net/10261/123567
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dc.contributor.author | Vidal, Roberto | - |
dc.contributor.author | Ginard, Daniel | - |
dc.contributor.author | Khorrami, Sam | - |
dc.contributor.author | Mora-Ruiz, Merit del Rocio | - |
dc.contributor.author | Muñoz, Raúl | - |
dc.contributor.author | Hermoso, Marcela A. | - |
dc.contributor.author | Díaz, Sara | - |
dc.contributor.author | Cifuentes, Ana | - |
dc.contributor.author | Orfila, Alejandro | - |
dc.contributor.author | Rosselló-Mora, Ramón | - |
dc.date.accessioned | 2015-10-20T08:54:17Z | - |
dc.date.available | 2015-10-20T08:54:17Z | - |
dc.date.issued | 2015-09-01 | - |
dc.identifier | doi: 10.1016/j.syapm.2015.06.008 | - |
dc.identifier | issn: 1618-0984 | - |
dc.identifier.citation | Systematic and Applied Microbiology 38(6): 442-452 (2015) | - |
dc.identifier.uri | http://hdl.handle.net/10261/123567 | - |
dc.description.abstract | © 2015 Elsevier GmbH. Next generation sequencing approaches allow the retrieval of several orders of magnitude larger numbers of amplified single sequences in 16S rRNA diversity surveys than classical methods. However, the sequences are only partial and thus lack sufficient resolution for a reliable identification. The OPU approach used here, based on a tandem combination of high quality 454 sequences (mean >500 nuc) applying strict OTU thresholds, and phylogenetic inference based on parsimony additions to preexisting trees, seemed to improve the identification yields at the species and genus levels. A total of thirteen biopsies of Crohn-diagnosed patients (CD) and seven healthy controls (HC) were studied. In most of the cases (73%), sequences were affiliated to known species or genera and distinct microbial patterns could be distinguished among the CD subjects, with a common depletion of Clostridia and either an increased presence of Bacteroidetes (CD1) or an anomalous overrepresentation of Proteobacteria (CD2). Faecalibacterium prausnitzii presence was undetectable in CD, whereas Bacteroides vulgatus-B. dorei characterized HC and some CD groups. Altogether, the results showed that a microbial composition with predominance of Clostridia followed by Bacteroidetes, with F. prausnitzii and B. vulgatus-B. dorei as major key bacteria, characterized what could be considered a balanced structure in HC. The depletion of Clostridia seemed to be a common trait in CD. | - |
dc.description.sponsorship | This work has been funded by Spanish Council project CSIC/UCHILE 02/11-12, by the Spanish Ministry of Economy projectCGL2012-39627-C03-03, and also with the help of the Competitive Research Groups (Microbiology) of the Government of the Balearic Islands, which were both also supported by European Regional Development Fund (FEDER) funds. Additional funding was obtained from the Chilean Ministry project FONDECYT1120557, the MEBICROHN project, and the preparatory phase of the Microbial Resource Research Infrastructure (MIRRI) funded by the EU (grant number 312251). MMR's PhD was supported by fellowship CVU 265934 from the National Council of Science and Technology (CONACyT), Mexico | - |
dc.publisher | Elsevier | - |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/312251 | - |
dc.rights | closedAccess | - |
dc.subject | Crohn’s disease | - |
dc.subject | Pyrotagging | - |
dc.subject | Operational phylogenetic units | - |
dc.subject | Gut microbiome | - |
dc.subject | Firmicutes | - |
dc.subject | Faecalibacterium prausnitzii | - |
dc.title | Crohn associated microbial communities associated to colonic mucosal biopsies in patients of the western Mediterranean | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1016/j.syapm.2015.06.008 | - |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.syapm.2015.06.008 | - |
dc.date.updated | 2015-10-20T08:54:19Z | - |
dc.description.version | Peer Reviewed | - |
dc.language.rfc3066 | eng | - |
dc.contributor.funder | Consejo Nacional de Ciencia y Tecnología (México) | - |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | - |
dc.contributor.funder | European Commission | - |
dc.contributor.funder | Govern de les Illes Balears | - |
dc.contributor.funder | Fondo Nacional de Desarrollo Científico y Tecnológico (Chile) | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003141 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100002850 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
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