English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/123567
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Crohn associated microbial communities associated to colonic mucosal biopsies in patients of the western Mediterranean

AuthorsVidal, Roberto; Ginard, Daniel; Khorrami, Sam; Mora-Ruiz, Merit del Rocio; Muñoz, Raúl; Hermoso, Marcela; Díaz, Sara; Cifuentes, Ana ; Orfila, Alejandro ; Rosselló-Mora, Ramón
KeywordsCrohn disease
Operational phylogenetic units
Gut microbiome
Faecalibacterium prausnitzii
Issue Date1-Sep-2015
CitationSystematic and Applied Microbiology 38(6): 442-452 (2015)
Abstract© 2015 Elsevier GmbH. Next generation sequencing approaches allow the retrieval of several orders of magnitude larger numbers of amplified single sequences in 16S rRNA diversity surveys than classical methods. However, the sequences are only partial and thus lack sufficient resolution for a reliable identification. The OPU approach used here, based on a tandem combination of high quality 454 sequences (mean >500 nuc) applying strict OTU thresholds, and phylogenetic inference based on parsimony additions to preexisting trees, seemed to improve the identification yields at the species and genus levels. A total of thirteen biopsies of Crohn-diagnosed patients (CD) and seven healthy controls (HC) were studied. In most of the cases (73%), sequences were affiliated to known species or genera and distinct microbial patterns could be distinguished among the CD subjects, with a common depletion of Clostridia and either an increased presence of Bacteroidetes (CD1) or an anomalous overrepresentation of Proteobacteria (CD2). Faecalibacterium prausnitzii presence was undetectable in CD, whereas Bacteroides vulgatus-B. dorei characterized HC and some CD groups. Altogether, the results showed that a microbial composition with predominance of Clostridia followed by Bacteroidetes, with F. prausnitzii and B. vulgatus-B. dorei as major key bacteria, characterized what could be considered a balanced structure in HC. The depletion of Clostridia seemed to be a common trait in CD.
Publisher version (URL)http://dx.doi.org/10.1016/j.syapm.2015.06.008
Identifiersdoi: 10.1016/j.syapm.2015.06.008
issn: 1618-0984
Appears in Collections:(IMEDEA) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.