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Título

A novel amperometric biosensor based on gold nanoparticles anchored on reduced graphene oxide for sensitive detection of L-lactate tumor biomarker

AutorAzzouzi, Sawsen; Rotariua, Lucian; Benito, Ana M. ; Maser, Wolfgang K. ; Ben Alib, Mounir; Bala, Camelia
Palabras claveReduced graphene oxide
Gold nanoparticles
NADH
L-lactate
Cancer biomarker
L-lactate dehydrogenase
Fecha de publicaciónmar-2015
EditorElsevier
CitaciónBiosensors and Bioelectronics (69) : 280-286 (2015)
ResumenIn this work, a novel amperometric biosensor based on gold nanoparticles anchored on reduced graphene oxide (RGO-AuNPs) and L-lactate dehydrogenase (LDH) was developed for the sensing of Llactate. Firstly, the RGO-AuNPs modified screen printed electrodes were tested for NADH detection showing a wide dynamic range and a low detection limit. Next, the biosensor was constructed by incorporating both enzyme and RGO-AuNPs in a sol gel matrix derived from tetrametoxysilane and methyltrimetoxysilane. The enzyme loading, working pH, and coenzyme concentration were optimized. The biosensor linearly responded to L-lactate in the range of 10 μM - 5 mM and showed a good specific sensitivity of 154 μA/mM·cm2 with a detection limit of 0.13 μM. This was accompanied by good reproducibility and operational stability. Tests on artificial serum proved that L-lactate can be determined practically without interferences from commonly interfering compounds such as urate, paracetamol and L-ascorbate. Our LDH/RGO-AuNPs/SPCE based biosensor thus performs as electrochemical device for the detection of L-lactate as a viable early cancer bio-marker.
Versión del editorhttp://dx.doi.org/10.1016/j.bios.2015.03.012
URIhttp://hdl.handle.net/10261/123523
DOI10.1016/j.bios.2015.03.012
ISSN0956-5663
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