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Title

In vitro transcription/translation system: A versatile tool in the search for missing proteins

AuthorsHorvatovich, Péter; Fuentes, Manuel ; Díez, Paula; González González, María; Ibarrola, Nieves ; De Las Rivas, Javier ; Corrales, Fernando J.; LaBaer, Joshua; Marko-Varga, György
Issue Date2015
PublisherAmerican Chemical Society
CitationJournal of Proteome Research 14(9): 3441-3451 (2015)
AbstractApproximately 18% of all human genes purported to encode proteins have not been directly evidenced at the protein level, according to the validation criteria established by neXtProt, and are considered to be >missing> proteins. One of the goals of the Chromosome-Centric Human Proteome Project (C-HPP) is to identify as many of these missing proteins as possible in human samples using mass spectrometry-based methods. To further this goal, a consortium of C-HPP teams (chromosomes 5, 10, 16, and 19) has joined forces to devise new strategies to identify missing proteins by use of a cell-free in vitro transcription/translation system (IVTT). The proposed strategy employs LC-MS/MS data-dependent acquisition (DDA) and targeted selective reaction monitoring (SRM) methods to scrutinize low-complexity samples derived from IVTT. The optimized assays are then applied to identify missing proteins in human cells and tissues. We describe the approach and show proof-of-concept results for development of LC-SRM assays for identification of 18 missing proteins. We believe that the IVTT system, when coupled with downstream mass spectrometric identification, can be applied to identify proteins that have eluded more traditional methods of detection.
Descriptionet al.
URIhttp://hdl.handle.net/10261/123255
DOI10.1021/acs.jproteome.5b00486
Identifiersdoi: 10.1021/acs.jproteome.5b00486
e-issn: 1535-3907
issn: 1535-3893
Appears in Collections:(IBMCC) Artículos
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