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Protein arrays: recent achievements and their application to study the human proteome

AutorCasado-Vela, Juan; González González, María; Matarraz, Sergio; Sayagués, José María ; Fuentes, Manuel ; Lacal, Juan Carlos
Fecha de publicación2013
EditorBentham Science Publishers
CitaciónCurrent Proteomics 10(2): 83-97 (2013)
ResumenProtein arrays represent a step forward in proteomic technologies, as they enable to address biological and functional questions by large-scale approaches (i.e., up to several thousand proteins may be monitored in a single experiment). Here we review recent achievements of protein arrays and their classification. We also summarize current and future applications of this technology to address the study of the human proteome including protein identification, bio-marker discovery, screening of protein-protein/molecule interactions, detection of protein post-translational modifications and serum profiling. Advantages and limitations of protein arrays compared to other proteomic technologies are also discussed here. The use, advances and recent applications of fluorescent microspheres as a promising alternative to planar arrays are also summarized in this report. Finally, we propose future perspectives of protein arrays from a comprehensive standpoint that includes technological, biological, economical and practical aspects. © 2013 Bentham Science Publishers.
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URIhttp://hdl.handle.net/10261/123227
DOI10.2174/1570164611310020003
Identificadoresissn: 1570-1646
e-issn: 1875-6247
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