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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/123162

Single mechanosensitive and ca(2+)-sensitive channel currents recorded from mouse and human embryonic stem cells

AutorSoria Escoms, Bernat ; Navas, Sergio ; Hmadcha, Abdelkrim ; Hamill, Owen P.
Palabras claveCalcium sensitive channels
Embryonic stem cells
Mechanosensitive channels
Fecha de publicación28-nov-2012
CitaciónJournal of Membrane Biology 246(3): 215-230 (2013)
ResumenCell-attached and inside-out patch clamp recording was used to compare the functional expression of membrane ion channels in mouse and human embryonic stem cells (ESCs). Both ESCs express mechanosensitive Ca(2+) permeant cation channels (MscCa) and large conductance (200 pS) Ca(2+)-sensitive K(+) (BK(Ca2+)) channels but with markedly different patch densities. MscCa is expressed at higher density in mESCs compared with hESCs (70 % vs. 3 % of patches), whereas the BK(Ca2+) channel is more highly expressed in hESCs compared with mESCs (~50 % vs. 1 % of patches). ESCs of both species express a smaller conductance (25 pS) nonselective cation channel that is activated upon inside-out patch formation but is neither mechanosensitive nor strictly Ca(2+)-dependent. The finding that mouse and human ESCs express different channels that sense membrane tension and intracellular [Ca(2+)] may contribute to their different patterns of growth and differentiation in response to mechanical and chemical cues.
Versión del editorhttp://dx.doi.org/10.1007/s00232-012-9523-6
Identificadoresdoi: 10.1007/s00232-012-9523-6
issn: 0022-2631
e-issn: 1432-1424
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