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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/123117
Título

In vitro characterization of a miR-122-sensitive double-helical switch element in the 5′ region of hepatitis C virus RNA

AutorDíaz-Toledano, Rosa; Ariza-Mateos, Ascensión; Birk, A.; Martínez-García, Belén; Gómez, Jordi
Fecha de publicaciónjul-2009
EditorOxford University Press
CitaciónNucleic Acids Research
ResumenIt has been proposed that the hepatitis C virus (HCV) internal ribosome entry site (IRES) resides within a locked conformation, owing to annealing of its immediate flanking sequences. In this study, structure probing using Escherichia coli dsRNA-specific RNase III and other classical tools showed that this region switches to an open conformation triggered by the liver-specific microRNA, miR-122. This structural transition, observed in vitro, may be the mechanistic basis for the involvement of downstream IRES structural domain VI in translation, as well as providing a role of liver-specific miR-122 in HCV infection. In addition, the induced RNA switching at the 5′ untranslated region could ultimately represent a new mechanism of action of micro-RNAs.
DescripciónSupplementari Data http://nar.oxfordjournals.org/content/suppl/2009/10/12/gkp553.DC1
Versión del editorhttp://nar.oxfordjournals.org/content/early/2009/07/03/nar.gkp553.full
URIhttp://hdl.handle.net/10261/123117
DOI10.1093/nar/gkp553
ISSN0305-1048
E-ISSN1362-4962
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