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In vitro characterization of a miR-122-sensitive double-helical switch element in the 5′ region of hepatitis C virus RNA

AuthorsDíaz-Toledano, Rosa ; Ariza-Mateos, Ascensión ; Birk, A.; Martínez-García, Belén ; Gómez, Jordi
Issue DateJul-2009
PublisherOxford University Press
CitationNucleic Acids Research
AbstractIt has been proposed that the hepatitis C virus (HCV) internal ribosome entry site (IRES) resides within a locked conformation, owing to annealing of its immediate flanking sequences. In this study, structure probing using Escherichia coli dsRNA-specific RNase III and other classical tools showed that this region switches to an open conformation triggered by the liver-specific microRNA, miR-122. This structural transition, observed in vitro, may be the mechanistic basis for the involvement of downstream IRES structural domain VI in translation, as well as providing a role of liver-specific miR-122 in HCV infection. In addition, the induced RNA switching at the 5′ untranslated region could ultimately represent a new mechanism of action of micro-RNAs.
DescriptionSupplementari Data http://nar.oxfordjournals.org/content/suppl/2009/10/12/gkp553.DC1
Publisher version (URL)http://nar.oxfordjournals.org/content/early/2009/07/03/nar.gkp553.full
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