English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/123112
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

PTTG2 silencing results in induction of epithelial-to-mesenchymal transition and apoptosis

AutorMéndez-Vidal, Cristina ; Gámez del Estal, María del Mar; Moreno-Mateos, Miguel A. ; Espina, Águeda G. ; Torres, Belén ; Pintor-Toro, José Antonio
Palabras clavePttg/securin
EMT
E-cadherin
Anoikis
Fecha de publicación7-mar-2013
EditorNature Publishing Group
CitaciónCell Death & Disease 4: e530 (2013)
ResumenHuman securin, also known as human pituitary tumor-transforming gene 1 (pttg1), plays a key role in cell-cycle regulation. Two homologous genes, pttg2 and pttg3, have been identified although very little is known about their physiological function. In this study, we aimed at the characterization of these two pttg1 homologs. Real-time PCR analysis using specific probes demonstrated that Pttg2 is expressed at very low levels in various cell lines and tissues whereas Pttg3 was largely undetectable. We focused on the study of Pttg2 and found that, unlike PTTG1, PTTG2 lacks transactivation activity and does not bind to separase, making improbable a role in the control of sister chromatids separation. To further investigate the biological role of pttg2, we used short hairpin RNA inhibition of Pttg2 and found that cells with reduced Pttg2 levels assumed a rounded morphology compatible with a defect in cell adhesion and died by apoptosis in a p53- and p21-dependent manner. Using microarray technology, we generated a gene expression profile of Pttg2-depleted cells versus wild-type cells and found that knockdown of PTTG2 results in concomitant downregulation of E-cadherin and elevated vimentin levels, consistent with EMT induction. The observation of aberrant cellular behaviors in Pttg2-silenced cells reveals functions for pttg2 in cell adhesion and provides insights into a potential role in cell invasion. © 2013 Macmillan Publishers Limited.
Versión del editorhttp://dx.doi.org/10.1038/cddis.2013.48
URIhttp://hdl.handle.net/10261/123112
DOI10.1038/cddis.2013.48
Identificadoresdoi: 10.1038/cddis.2013.48
issn: 2041-4889
Aparece en las colecciones: (CABIMER) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
PTTG2_silencing_results_MendezVidal.pdf2,63 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.