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Título: | Heteromers of amyloid precursor protein in cerebrospinal fluid |
Autor: | Cuchillo-Ibáñez, Inmaculada CSIC ORCID; López-Font, Inmaculada CSIC ORCID; Boix-Amorós, Alba CSIC ORCID; Brinkmalm, Gunnar; Blennow, Kaj; Molinuevo, José Luis; Sáez-Valero, Javier CSIC ORCID | Palabras clave: | sAPPα sAPPβ Heteromers Cerebrospinal fluid Alzheimer’s disease ELISA |
Fecha de publicación: | 8-ene-2015 | Editor: | BioMed Central | Citación: | Molecular Neurodegeneration 10(2): 1-11(2015) | Resumen: | Background: Soluble fragments of the amyloid precursor protein (APP) generated by α- and β-secretases, sAPPα
and sAPPβ, have been postulated as promising new cerebrospinal fluid (CSF) biomarkers for the clinical diagnosis of
Alzheimer’s disease (AD). However, the capacity of these soluble proteins to assemble has not been explored and
could be relevant. Our aim is to characterize possible sAPP oligomers that could contribute to the quantification of
sAPPα and sAPPβ in CSF by ELISA, as well as to characterize the possible presence of soluble full-length APP
(sAPPf). Results: We employed co-immunoprecipitation, native polyacrylamide gel electrophoresis and ultracentrifugation in sucrose density gradients to characterize sAPP oligomers in CSF. We have characterized the presence of sAPPf in CSF from NDC and AD subjects and demonstrated that all forms, including sAPPα and sAPPβ, are capable of assembling into heteromers, which differ from brain APP membrane-dimers. We measured sAPPf, sAPPα and sAPPβ by ELISA in CSF samples from AD (n = 13) and non-disease subjects (NDC, n = 13) before and after immunoprecipitation with antibodies against the C-terminal APP or against sAPPβ. We demonstrated that these sAPP heteromers participate in the quantification of sAPPα and sAPPβ by ELISA. Immunoprecipitation with a C-terminal antibody to remove sAPPf reduced by ~30% the determinations of sAPPα and sAPPβ by ELISA, whereas immunoprecipitation with an APPβ antibody reduced by ~80% the determination of sAPPf and sAPPα. Conclusions: The presence of sAPPf and sAPP heteromers should be taken into consideration when exploring the levels of sAPPα and sAPPβ as potential CSF biomarkers. |
Versión del editor: | http://dx.doi.org/10.1186/1750-1326-10-2 | URI: | http://hdl.handle.net/10261/122961 | DOI: | 10.1186/1750-1326-10-2 | ISSN: | 1750-1326 |
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