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Balanced production of ribosome components is required for proper G/S transition in saccharomyces cerevisiae

AuthorsGómez-Herreros, Fernando ; Rodríguez-Galán, Olga ; Morillo-Huesca, Macarena ; Maya, Douglas; Arista-Romero, María; Cruz, Jesús de la; Chávez, Sebastián; Muñoz-Centeno, Mari Cruz
KeywordsCell Cycle
Ribosomal RNA (rRNA)
Ribosome Assembly
RNA Polymerase I
RNA Polymerase II
RNA Polymerase III
G1/S Transition
Free Ribosomal Proteins
Issue Date16-Sep-2013
PublisherAmerican Society for Biochemistry and Molecular Biology
CitationJournal of Biological Chemistry 288(44): 31689- 31700 (2013)
AbstractCell cycle regulation is a very accurate process that ensures cell viability and the genomic integrity of daughter cells. A fundamental part of this regulation consists in the arrest of the cycle at particular points to ensure the completion of a previous event, to repair cellular damage, or to avoid progression in potentially risky situations. In this work, we demonstrate that a reduction in nucleotide levels or the depletion of RNA polymerase I or III subunits generates a cell cycle delay at the G1/S transition in Saccharomyces cerevisiae. This delay is concomitant with an imbalance between ribosomal RNAs and proteins which, among others, provokes an accumulation of free ribosomal protein L5. Consistently with a direct impact of free L5 on the G1/S transition, rrs1 mutants, which weaken the assembly of L5 and L11 on pre-60S ribosomal particles, enhance both the G1/S delay and the accumulation of free ribosomal protein L5. We propose the existence of a surveillance mechanism that couples the balanced production of yeast ribosomal components and cell cycle progression through the accumulation of free ribosomal proteins. This regulatory pathway resembles the p53-dependent nucleolar-stress checkpoint response described in human cells, which indicates that this is a general control strategy extended throughout eukaryotes. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.
Publisher version (URL)http://dx.doi.org/10.1074/jbc.M113.500488
Identifiersdoi: 10.1074/jbc.M113.500488
issn: 0021-9258
e-issn: 1083-351X
Appears in Collections:(CABIMER) Artículos
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