English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/122713
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Separation, quantitative determination, and fatty acid profiling of monoglycerides in fatty-acid methyl esters (FAME) using an online, hyphenated technique based on AMD-FDIC-ESI-MS

AutorCebolla, Vicente L. ; Jarne, Carmen; Membrado, Luis; Savirón, María; Orduna, Jesús
Fecha de publicación2014
CitaciónHPTLC 2014
ResumenFAME is a biodiesel obtained from vegetal or animal oils through esterification and transesterification processes, which is used as a total or partial substitute for petroleum derived‐diesel. Monoacylglycerides are one class of the impurities coming from an incomplete esterification reaction. Due to their high fusion point and low solubility at low temperatures, monoacylglycerides can produce deposits in motor engines. The maximum concentration of monoacylglycerides tolerated in FAME by UNE‐EN14214:2013 standard is 0.8 wt%. We evaluate here the determination by HPTLC of monoacylglycerides in 9 FAMEs from vegetal and animal origins. Monoglycerides have been separated from other FAME components (fatty acids, diglycerides, triglycerides and fatty acid‐methyl esters) using a 3‐step AMD separation based on a t‐butyl methyl ether – dichloromethane ‐ n‐heptane gradient, over a total migration step of 90 mm, in one hour. Densitometric Fluorescence Detection by Intensity Changes and quantitative determination of monoacylglycerides have been carried out by plate post‐impregnation using primuline (200 ppm) by excitation at 365 nm. Identification of monoacylglycerides was done in FDIC by using glyceryl stearate as standard, and also by ESI‐MS, after an on‐line extraction of the peak using a head‐elution based TLC‐MS interface. Moreover ESI‐MS provides the fatty acid profile of the corresponding FAME‐derived monoglyceride. One plate per analyzed FAME was used. In each plate, the corresponding FAME was applied on triplicate (three 12 mm‐bands, 2500 g each), together with 10 sample bands corresponding to different sample loads of the standard. Intra‐plate and inter‐plate calibrations show adequate repeatability, and a good sensitivity to determine monoacylglycerides far below the limit required by the UNE standard.
DescripciónResumen del trabajo presentado al: "22nd International Symposium High-Performance Thin-Layer Chromatography" celebrado en Lyon (Francia) del 2 al 4 de julio de 2014.
Aparece en las colecciones: (ICB) Comunicaciones congresos
(ISQCH) Comunicaciones congresos
(ICMA) Comunicaciones congresos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Mostrar el registro completo

NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.