English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/12263
Compartir / Impacto:
Add this article to your Mendeley library MendeleyBASE
Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL

Choline kinase alpha depletion selectively kills tumoral cells

AutorBáñez Coronel, Mónica; Ramírez de Molina, Ana; Rodríguez-González, Agustín; Sarmentero, Jacinto; Ramos, Mª Angeles; García Cabezas, Miguel Ángel; Garcia-Oroz, Lourdes; Lacal, Juan Carlos
Palabras claveCholine Kinase
Anticancer drugs
Target validation
ChoK Inhibitors
Fecha de publicacióndic-2008
EditorBentham Science Publishers
CitaciónCurrent Cancer Drug Targets. 2008 Dec;8(8):709-19
ResumenCholine Kinase (ChoK) comprises a family of cytosolic enzymes involved in the synthesis of phosphatidylcholine (PC), the most abundant phospholipid in eukaryotic cell membranes. One of the ChoK isoforms, Choline Kinase α (ChoKα), is found over expressed in human tumours. Chemical inhibitors able to interfere with ChoK activity have proven to be effective antitumoral drugs in vitro and in vivo. To validate the use of selective ChoKα inhibitors in cancer therapy, we have developed a genetic strategy to interfere specifically with ChoKα activity based on the generation of a shRNA against the alpha isoform of ChoK. Here we demonstrate that specific inhibition of ChoKα by shRNA has antitumor activity. The specific depletion of ChoKα induces apoptosis in several tumor-derived cell lines from breast, bladder, lung and cervix carcinoma tumors, while the viability of normal primary cells is not affected. Furthermore, this selective antiproliferative effect is achieved both under in vitro and in vivo conditions, as demonstrated by an inducible ChoKα suppression system in human tumour xenografts. These results demonstrate that ChoKα inhibition is a useful antitumoral strategy per se, and provides definitive and non-ambiguous evidence that ChoKα can be used as an efficient and selective drug target for cancer therapy
Versión del editorhttp://www.benthamdirect.org/pages/content.php?CCDT/2008/00000008/00000008/0007U
Aparece en las colecciones: (CNB) Artículos
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro completo

NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.