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Systematic HPLC/ESI-High Resolution-qTOF-MS Methodology for Metabolomic Studies in Nonfluorescent Chlorophyll Catabolites Pathway

AutorRíos, José Julián; Roca, María ; Pérez Gálvez, Antonio
Fecha de publicación12-ene-2015
EditorHindawi Publishing Corporation
CitaciónJournal of Analytical Methods in Chemistry 2015 (2015)
ResumenCharacterization of nonfluorescent chlorophyll catabolites (NCCs) and dioxobilane-type nonfluorescent chlorophyll catabolite (DNCC) in peel extracts of ripened lemon fruits (Citrus limon L.) was performed by HPLC/ESI-high resolution-qTOF-MS method. Compounds were identified in samples on the basis of measured accurate mass, isotopic pattern, and characteristic fragmentation profile with an implemented software postprocessing routine. Three NCC structures already identified in other vegetal tissues were present in the lemon fruit peels (Cl-NCC1; Cl-NCC2; Cl-NCC4) while a new structure not defined so far was characterized (Cl-NCC3). This catabolite exhibits an exceptional arrangement of the peripheral substituents, allowing concluding that the preferences for the NCC modifications could be a species-related matter.
Descripción11 páginas; 2 tablas; 3 figuras; 2 esquemas
Versión del editorhttp://dx.doi.org/10.1155/2015/490627
URIhttp://hdl.handle.net/10261/122588
DOI10.1155/2015/490627
ISSN2090-8865
E-ISSN2090-8873
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