English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/122486
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

The striking and unexpected cytogenetic diversity of genus Tanacetum L. (Asteraceae): A cytometric and fluorescent in situ hybridisation study of Iranian taxa

AutorOlanj, Nayyereh; Garnatje, Teresa ; Sonboli, Ali; Vallès, Joan; Garcia, Sònia
Palabras clave5S
35S
Aneuploidy
Evolutionary cytogenetics
Genomic instability
L-type arrangement
Polyploidy
Odd ploidy
Ribosomal DNA
Fecha de publicación8-jul-2015
EditorBioMed Central
CitaciónBMC Plant Biology 15: 174 (2015)
Resumen[Background] Although karyologically well studied, the genus Tanacetum (Asteraceae) is poorly known from the perspective of molecular cytogenetics. The prevalence of polyploidy, including odd ploidy warranted an extensive cytogenetic study. We studied several species native to Iran, one of the most important centres of diversity of the genus. We aimed to characterise Tanacetum genomes through fluorochrome banding, fluorescent in situ hybridisation (FISH) of rRNA genes and the assessment of genome size by flow cytometry. We appraise the effect of polyploidy and evaluate the existence of intraspecific variation based on the number and distribution of GC-rich bands and rDNA loci. Finally, we infer ancestral genome size and other cytogenetic traits considering phylogenetic relationships within the genus.
[Results] We report first genome size (2C) estimates ranging from 3.84 to 24.87 pg representing about 11 % of those recognised for the genus. We found striking cytogenetic diversity both in the number of GC-rich bands and rDNA loci. There is variation even at the population level and some species have undergone massive heterochromatic or rDNA amplification. Certain morphometric data, such as pollen size or inflorescence architecture, bear some relationship with genome size. Reconstruction of ancestral genome size, number of CMA+ bands and number of rDNA loci show that ups and downs have occurred during the evolution of these traits, although genome size has mostly increased and the number of CMA+ bands and rDNA loci have decreased in present-day taxa compared with ancestral values.
[Conclusions]Tanacetum genomes are highly unstable in the number of GC-rich bands and rDNA loci, although some patterns can be established at the diploid and tetraploid levels. In particular, aneuploid taxa and some odd ploidy species show greater cytogenetic instability than the rest of the genus. We have also confirmed a linked rDNA arrangement for all the studied Tanacetum species. The labile scenario found in Tanacetum proves that some cytogenetic features previously regarded as relatively constant, or even diagnostic, can display high variability, which is better interpreted within a phylogenetic context.
Versión del editorhttp://dx.doi.org/10.1186/s12870-015-0564-8
URIhttp://hdl.handle.net/10261/122486
DOI10.1186/s12870-015-0564-8
ISSN1471-2229
Identificadoresissn: 1471-2229
Aparece en las colecciones: (IBB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Striking-and-unexpected-cytogenetic-diversity-genus-tanacetum-Garnatje-Teresa.pdf3,18 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.