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dc.contributor.authorGüimil García, Ramónes_ES
dc.contributor.authorBrank, Adam S.es_ES
dc.contributor.authorChristman, Judith K.es_ES
dc.contributor.authorMárquez, Víctor E.es_ES
dc.contributor.authorEritja Casadellà, Ramónes_ES
dc.date.accessioned2015-09-16T06:32:42Z-
dc.date.available2015-09-16T06:32:42Z-
dc.date.issued2001-
dc.identifier.citationAntisense and Nucleic Acid Drug Developmentes_ES
dc.identifier.urihttp://hdl.handle.net/10261/122143-
dc.description.abstractThe incorporation of 5-azacytosine residues into DNA causes potent inhibition of DNA (Cytosine-C5) methyltransferases. The synthesis of oligodeoxyribonucleotides incorporating single or multiple 5-aza-2′-deoxycytidine residues at precise sites was undertaken to generate an array of sequences containing the reactive 5-azacytosine base as specific target sites for enzymatic methylation. Preparation of these modified oligonucleotides requires the use of 2-(p-nitrophenyl)ethyloxycarbonyl (NPEOC) groups for the protection of exocyclic amino functions. These groups are removed under mild conditions, thus avoiding conventional protocols that are detrimental to the integrity of the 5-azacytosine ring.es_ES
dc.description.sponsorshipa European Molecular Biology Laboratory, D-69117 Heidelberg, Germany b Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE 69198-4525, United States c UNMC/Eppley Cancer Center, University of Nebraska Medical Center, Omaha, NE 69198-4525, United States d Laboratory of Medicinal Chemistry, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, MD 21702, United States e Institut de Biología Molecular de Barcelona, CSIC, Jordi Girona 18-26, E-08034 Barcelona, Spaines_ES
dc.language.isoenges_ES
dc.publisherMary Ann Liebertes_ES
dc.relation.isversionofPreprintes_ES
dc.rightsopenAccesses_ES
dc.subjectcytosine derivativees_ES
dc.subjectDNAes_ES
dc.subjectoligonucleotidees_ES
dc.subjectmethyltransferase inhibitores_ES
dc.titleSynthesis of oligonucleotide inhibitors of DNA (Cytosine-C5) methyltransferase containing 5-azacytosine residues at specific siteses_ES
dc.typeartículoes_ES
dc.description.peerreviewedNoes_ES
dc.relation.publisherversiondoi:10.1089/108729001753411335es_ES
dc.identifier.e-issn10872906-
dc.relation.csices_ES
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