English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/122143
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Synthesis of oligonucleotide inhibitors of DNA (Cytosine-C5) methyltransferase containing 5-azacytosine residues at specific sites

AuthorsGüimil García, Ramón; Brank, Adam S.; Christman, Judith K.; Márquez, Víctor E.; Eritja Casadellà, Ramón
Keywordscytosine derivative
DNA
oligonucleotide
methyltransferase inhibitor
Issue Date2001
PublisherMary Ann Liebert
CitationAntisense and Nucleic Acid Drug Development
AbstractThe incorporation of 5-azacytosine residues into DNA causes potent inhibition of DNA (Cytosine-C5) methyltransferases. The synthesis of oligodeoxyribonucleotides incorporating single or multiple 5-aza-2′-deoxycytidine residues at precise sites was undertaken to generate an array of sequences containing the reactive 5-azacytosine base as specific target sites for enzymatic methylation. Preparation of these modified oligonucleotides requires the use of 2-(p-nitrophenyl)ethyloxycarbonyl (NPEOC) groups for the protection of exocyclic amino functions. These groups are removed under mild conditions, thus avoiding conventional protocols that are detrimental to the integrity of the 5-azacytosine ring.
Publisher version (URL)doi:10.1089/108729001753411335
URIhttp://hdl.handle.net/10261/122143
E-ISSN10872906
Appears in Collections:(IQAC) Artículos
Files in This Item:
File Description SizeFormat 
antisense2001.pdf162,4 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.