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Título

Rapid production of fertile transgenic plants of rye (Secale cereale L.)

AutorCastillo Alonso, Ana María ; Vasil, Vimla; Vasil, Indra K.
Fecha de publicación1994
EditorNature Publishing Group
CitaciónNature Biotechnology 12: 1366-1371 (1994)
ResumenWe have produced transgenic rye plants by the direct delivery of plasmid DNA, containing the uidA and bar genes, into young embryogenic calli using high velocity microprojectiles. Of the fourteen independent callus lines selected on varying concentrations of phosphinothricin (PPT), the active ingredient of the herbicide Basta, six showed phosphinothricin acetyltransferase (PAT) activity. Plants (R0) were regenerated from two putative transformed lines on medium containing Basta. All of the R0 plants recovered from the two lines showed GUS expression (in leaves, roots, pistils and pollen), PAT activity and resistance to topical application of Basta. The presence of the uidA and bar genes and their integration into nuclear DNA in transformed R0 plants and their R1 progeny was confirmed by Southern analysis. Flowering fertile R0 plants were obtained 8–9 months after the initial culture and bombardment. Male and female transmission of the two genes, which segregated as dominant Mendelian traits in R1 plants, was demonstrated.
Descripción6 Pags.
Versión del editorhttp://dx.doi.org/10.1038/nbt1294-1366
URIhttp://hdl.handle.net/10261/122022
DOI10.1038/nbt1294-1366
ISSN1087-0156
E-ISSN1546-1696
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