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An Improved HPLC method for rapid analysis of the xanthophyll cycle pigments

AuthorsVal Falcón, Jesús ; Monge Pacheco, Emilio ; Baker, Neil, R.
Issue Date1994
PublisherOxford University Press
CitationJournal of Chromatographic Science 32 (7): 286-289 (1994)
AbstractA new high-performance liquid chromatographic (HPLC) method for the rapid separation of photosynthetic pigments of higher plants is reported. The method separates zeaxanthin from lutein with a resolution power (Rs) of 1.77. This method uses only two isocratic phases and employs a 10-cm C18 column with a flow rate of 2 mL/min. Consequently, simple HPLC systems can be used for analyses of the pigments, which can be chromatographed in a total time of 13 min. Routine and rapid analysis of the xanthophyll pigments, which were recently implicated in the photoinhibition of photosynthesis, can be achieved with this method.
Description4 Pags.
Publisher version (URL)http://dx.doi.org/10.1093/chromsci/32.7.286
Appears in Collections:(EEAD) Artículos
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